BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16267

Title: Solution NMR structure of tungsten formylmethanofuran dehydrogenase subunit D from Archaeoglobus fulgidus, Northeast Structural Genomics Consortium target AtT7

Deposition date: 2009-04-28 Original release date: original

Authors: Eletsky, Alexander; ., Wu; ., .; ., .; ., .; 7, .; Thomas, 8

Citation: Eletsky, Alexander; ., Wu; ., .; ., .; ., .; 16267, .; 8, 16267; , . "Solution NMR structure of tungsten formylmethanofuran dehydrogenase subunit D from Archaeoglobus fulgidus"  Proteins: Struct. Funct. Genet. ., .-..

Assembly members:
AtT7_protein, polymer, 146 residues, 16169.288 Da.

Natural source:   Common Name: not available   Taxonomy ID: 2234   Superkingdom: not available   Kingdom: Archaeoglobus   Genus/species: fulgidus VC-16

Experimental source:   Production method: recombinant technology' 'Escherichia coli' . . . Escherichia coli 'BL21 DE3 gold magic' . . . . . 'p15Tv lic

Entity Sequences (FASTA):
AtT7_protein: MGSSHHHHHHSSGRENLYFQ GHMLEVEVISGRTLNQGATV EEKLTEEYFNAVNYAEINEE DWNALGLQEGDRVKVKTEFG EVVVFAKKGDVPKGMIFIPM GPYANMVIDPSTDGTGMPQF KGVKGTVEKTDEKVLSVKEL LEAIGS

Data sets:
Data typeCount
13C chemical shifts604
1421H chemical shifts

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AtT7_protein1

Entities:

Entity 1, AtT7_protein 146 residues - 16169.288 Da.

Residues 1-23 and 145-146 represent non-native affinity tags

1   MET.162675.HIS.1626711.
2   GLY.1626717.PHE.1626723.
3   GLU.1626729.GLY.1626735.
4   GLY.1626741.LYS.1626747.
5   PHE.1626753.ALA.1626759.
6   ASP.1626765.LEU.1626771.
7   VAL.1626777.PHE.1626783.
8   PHE.1626789.VAL.1626795.
9   PHE.16267101.TYR.16267107.
10   ASP.16267113.THR.16267119.
11   LYS.16267125.VAL.16267131.
12   LYS.16267137.GLU.16267143.
13   GLY.

Samples:

NC: AtT7 protein' '[U-100% 13C; U-100% 15N]; .; .; . 16267; . 6; ., 10,

NC5: AtT7 protein' '[U-7% 13C; U-100% 15N]; .; .; . 16267; . 6; ., 10,

NC5_phage: AtT7 protein' '[U-7% 13C; U-100% 15N]; .; .; . 16267; ., 13.25, 16267 – 6 H2O; 86 16267 natural abundance; .

sample_conditions_1: ionic strength: 300 mM; 7.0: . 16267; .: atm temperature; K: 16267

phage: ionic strength: 200 mM; 7.0: . 16267; .: atm temperature; K: 16267

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCNCisotropicsample_conditions_1
nosotropicnot availablenot available
not availablenot availablenot availablenot available
not availablenot available1not available
not availablenot available$sample_conditions_1not available
not availablesample_conditions_1not available750
not availablenot availablenot availablenot available
not availablenot availablenot availablenot available
not availablenot available1not available
not availablenot available$750not available
not available750not availablenot available
not availablenot availablenot availablenot available
1not availablenot availablenot available
$NCnot availablenot availablenot available
isotropicnot availablenot availablenot available
not availablenot availablenot availablenot available
not availablenot availablenot availablenot available
1not availablenot availablenot available
$sample_conditions_1not availablenot availablenot available
not availablenot availablenot availablenot available
not availablenot availablenot availablenot available

Software:

VNMRJ v2.1B, Varian - collection

TOPSPIN, Bruker Biospin - collection, 16267

PROSA v6.0.2, Guntert - processing

CARA v1.8.4, Keller and Wuthrich - chemical shift assignment, 16267

AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

CSI v2.0, Wishart and Sykes - data analysis

TALOS v2007.068.09.07, Shen, Cornilescu, Delaglio and Bax - data analysis

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

CNS v1.2.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

AutoStruct v2.1.1, Huang, Tejero, Powers and Montelione - structure validation

PSVS v1.3, Bhattacharya and Montelione - structure validation

NMR spectrometers:

  • Varian INOVA 750 MHz
  • Varian INOVA 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
AAB89327 .
. Formylmethanofuran dehydrogenase subunit D [Archaeoglobus fulgidus DSM 8774]
tRNA CCA-pyrophosphorylase [Archaeoglobus fulgidus] .

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts