BMRB Entry 16263

Name: Backbone and sidechain 1H, 15N and 13C assignments of the NLRP7 Pyrin domain
Published: 2009-07-23

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PDB ID: 2km6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16263
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and sidechain 1H, 15N and 13C assignments of the NLRP7 Pyrin domain

Deposition date:

2009-04-24

Original release date:

2009-07-23

Authors:

Pinheiro, Anderson; Ehart, Angela; Ebner, Nina; Schwarzenbacher, Robert; Peti, Wolfgang

Citation:

Citation: de Sa Pinheiro, Anderson; Ehart, Angela; Ebner, Nina; Proell, Martina; Schwarzenbacher, Robert; Peti, Wolfgang. "Backbone and sidechain (1)H, (15)N and (13)C assignments of the NLRP7 pyrin domain." Biomol. NMR Assignments 3, 207-209 (2009).
PubMed: 19888692

Assembly members:

Assembly members:
NLRP7_Pyd, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHis parallel

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NLRP7_Pyd: GHMTSPQLEWTLQTLLEQLN EDELKSFKSLLWAFPLEDVL QKTPWSEVEEADGKKLAEIL VNTSSENWIRNATVNILEEM NLTELCKMAKAEMMEDGQLE HHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	417
15N chemical shifts	101
1H chemical shifts	713

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	subunit_1	1

Entities:

Entity 1, subunit_1 106 residues - Formula weight is not available

Residues 1 and 2 (GH) are part of the protease cleavage site used to cleave off the solubility tag used in the expression of this protein. Residues 101-106 comprise a His6-tag used for purification.

1

GLY

HIS

MET

THR

SER

PRO

GLN

LEU

GLU

TRP

2

THR

LEU

GLN

THR

LEU

GLU

GLN

LEU

ASN

3

GLU

ASP

GLU

LEU

LYS

SER

PHE

LYS

SER

LEU

4

LEU

TRP

ALA

PHE

PRO

LEU

GLU

ASP

VAL

LEU

5

GLN

LYS

THR

PRO

TRP

SER

GLU

VAL

GLU

6

ALA

ASP

GLY

LYS

LEU

ALA

GLU

ILE

LEU

7

VAL

ASN

THR

SER

GLU

ASN

TRP

ILE

ARG

8

ASN

ALA

THR

VAL

ASN

ILE

LEU

GLU

MET

9

ASN

LEU

THR

GLU

LEU

CYS

LYS

MET

ALA

LYS

10

ALA

GLU

MET

GLU

ASP

GLY

GLN

LEU

GLU

11

HIS

Samples:

sample_1: NLRP7 Pyd, [U-100% 15N], 1.0 mM; sodium phosphate 20 mM; sodium chloride 50 mM; TCEP 0.25 mM; H2O 90%; D2O 10%

sample_2: NLRP7 Pyd, [U-100% 13C; U-100% 15N], 1.0 mM; sodium phosphate 20 mM; sodium chloride 50 mM; TCEP 0.25 mM; H2O 90%; D2O 10%

sample_3: NLRP7 Pyd 1.0 mM; sodium phosphate 20 mM; sodium chloride 50 mM; TCEP 0.25 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HNCACO	sample_2	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_2	isotropic	sample_conditions_1
3D C(CO)NH	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_3	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_3	isotropic	sample_conditions_1
2D DQF-COSY	sample_3	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Avance II 500 MHz

PDB	2KM6
DBJ	BAG63894
GB	AAI09125 AAI09126 AAL69963 AAO18158 AIC57936
REF	NP_001120727 NP_631915 NP_996611 XP_004061498 XP_006723138
SP	Q8WX94
TPG	DAA01246
AlphaFold	Q8WX94