BMRB Entry 16261

Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16261

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Title:
1H, 15N, 13C assignments of the Riphicephalus (Boophilus) microplus antimicrobial protein microplusin
Deposition date:
2009-04-20
Original release date:
2009-07-22
Authors:
Pires, Jose; Rezende, Carlos; Dias, Fernanda; Daffre, Sirlei
Citation:

Citation: Rezende, Carlos; Silva, Fernanda; Daffre, Sirlei; Pires, Jose. "(1)H, (15)N and (13)C assignments of the Rhipicephalus (Boophilus) microplus anti-microbial peptide microplusin."  Biomol. NMR Assignments 3, 187-189 (2009).
PubMed: 19888687

Assembly members:

Assembly members:
Microplusin, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: southern cattle tick   Taxonomy ID: 6941   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rhipicephalus microplus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSET-A (Invitrogen)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Microplusin: HHQELCTKGDDALVTELECI RLRISPETNAAFDNAVQQLN CLNRACAYRKMCATNNLEQA MSVYFTNEQIKEIHDAATAC DPEAHHEHDH

Data sets:
Data typeCount
13C chemical shifts254
15N chemical shifts99
1H chemical shifts493

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Microplusin1

Entities:

Entity 1, Microplusin 90 residues - Formula weight is not available

1   HISHISGLNGLULEUCYSTHRLYSGLYASP
2   ASPALALEUVALTHRGLULEUGLUCYSILE
3   ARGLEUARGILESERPROGLUTHRASNALA
4   ALAPHEASPASNALAVALGLNGLNLEUASN
5   CYSLEUASNARGALACYSALATYRARGLYS
6   METCYSALATHRASNASNLEUGLUGLNALA
7   METSERVALTYRPHETHRASNGLUGLNILE
8   LYSGLUILEHISASPALAALATHRALACYS
9   ASPPROGLUALAHISHISGLUHISASPHIS

Samples:

sample_1: Microplusin 0.95 mM; H2O 90%; D2O 10%

sample_2: Microplusin 0.95 mM; D2O 100%

sample_3: Microplusin, [U-15N], 0.52 mM; H2O 90%; D2O 10%

sample_4: Microplusin, [U-13C; U-15N], 0.35 mM; H2O 90%; D2O 10%

sample_5: Microplusin, [U-13C; U-15N], 0.35 mM; D2O 100%

sample_conditions_1: ionic strength: 50 mM; pH: 5.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
2D 1H-13C HMQCsample_5isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_3isotropicsample_conditions_1
3D CBCA(CO)NHsample_4isotropicsample_conditions_1
3D HNCACBsample_4isotropicsample_conditions_1
3D HBHA(CO)NHsample_4isotropicsample_conditions_1
3D H(CCO)NHsample_4isotropicsample_conditions_1
3D C(CO)NHsample_4isotropicsample_conditions_1
3D 1H-13C NOESYsample_5isotropicsample_conditions_1
3D HCCH-COSYsample_5isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker Avance III 800 MHz

Related Database Links:

PDB
GB AAO48942
SP Q86LE5
AlphaFold Q86LE5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks