BMRB Entry 16259

Name: Beta7 Integrin Cytoplasmic Tail 1H and 15N Chemical Shift Assignments
Published: 2009-12-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16259

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Beta7 Integrin Cytoplasmic Tail 1H and 15N Chemical Shift Assignments

Deposition date:

2009-04-17

Original release date:

2009-12-11

Authors:

Memo, Massimiliano; Anthis, Nicholas; Campbell, Iain

Citation:

Citation: Anthis, Nicholas; Haling, Jacob; Oxley, Camilla; Memo, Massimiliano; Wegener, Kate; Lim, Chinten; Ginsberg, Mark; Campbell, Iain. "Beta integrin tyrosine phosphorylation is a conserved mechanism for regulating talin-induced integrin activation." J. Biol. Chem. 284, 36700-36710 (2009).
PubMed: 19843520

Assembly members:

Assembly members:
beta7_integrin, polymer, 54 residues, 6446 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta7_integrin: GPRLSVEIYDRREYSRFEKE QQQLNWKQDSNPLYKSAITT TINPRFQEADSPTL

Data sets:

assigned_chemical_shifts
- pH-4.5
- pH-6.1

Data type	Count
15N chemical shifts	99
1H chemical shifts	39

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	beta7 integrain tail	1

Entities:

Entity 1, beta7 integrain tail 54 residues - 6446 Da.

1

GLY

PRO

ARG

LEU

SER

VAL

GLU

ILE

TYR

ASP

2

ARG

GLU

TYR

SER

ARG

PHE

GLU

LYS

GLU

3

GLN

LEU

ASN

TRP

LYS

GLN

ASP

SER

4

ASN

PRO

LEU

TYR

LYS

SER

ALA

ILE

THR

5

THR

ILE

ASN

PRO

ARG

PHE

GLN

GLU

ALA

ASP

6

SER

PRO

THR

LEU

Samples:

pH_6.1: D2O, [U-2H], 5%; sodium phosphate 50 mM; sodium chloride 100 mM; DTT 1 mM; DSS 0.1 mM; beta7 integrin, [U-15N], 0.05 mM; H2O 95%

pH_4.5: D2O, [U-2H], 5%; sodium acetate 20 mM; sodium azide 0.02%; DSS 0.1 mM; beta7 integrin, [U-15N], 0.2 mM; H2O 95%

pH6.1: ionic strength: 200 mM; pH: 6.1; pressure: 1 atm; temperature: 298 K

pH4.5: ionic strength: 10 mM; pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	pH_6.1	isotropic	pH6.1
3D 1H-15N NOESY	pH_4.5	isotropic	pH4.5
3D 1H-15N TOCSY	pH_4.5	isotropic	pH4.5
2D 1H-15N HSQC	pH_4.5	isotropic	pH4.5

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

Omega Spectrometer Operating Software vBeta 6.0.3b2, GE - collection

ANALYSIS, Boucher and Stevens - chemical shift assignment

NMR spectrometers:

GE OMEGA 600 MHz
GE OMEGA 950 MHz

PDB	2BRQ
DBJ	BAG10580 BAH12742
GB	AAA36118 AAA59184 AAA59185 AAB21332 AAB23688
REF	NP_000880 XP_001087864 XP_001141622 XP_003831078 XP_004053261
SP	P26010
AlphaFold	P26010