BMRB Entry 16259

Title:
Beta7 Integrin Cytoplasmic Tail 1H and 15N Chemical Shift Assignments
Deposition date:
2009-04-17
Original release date:
2009-12-11
Authors:
Memo, Massimiliano; Anthis, Nicholas; Campbell, Iain
Citation:

Citation: Anthis, Nicholas; Haling, Jacob; Oxley, Camilla; Memo, Massimiliano; Wegener, Kate; Lim, Chinten; Ginsberg, Mark; Campbell, Iain. "Beta integrin tyrosine phosphorylation is a conserved mechanism for regulating talin-induced integrin activation."  J. Biol. Chem. 284, 36700-36710 (2009).
PubMed: 19843520

Assembly members:

Assembly members:
beta7_integrin, polymer, 54 residues, 6446 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta7_integrin: GPRLSVEIYDRREYSRFEKE QQQLNWKQDSNPLYKSAITT TINPRFQEADSPTL

Data sets:
Data typeCount
15N chemical shifts99
1H chemical shifts39

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1beta7 integrain tail1

Entities:

Entity 1, beta7 integrain tail 54 residues - 6446 Da.

1   GLYPROARGLEUSERVALGLUILETYRASP
2   ARGARGGLUTYRSERARGPHEGLULYSGLU
3   GLNGLNGLNLEUASNTRPLYSGLNASPSER
4   ASNPROLEUTYRLYSSERALAILETHRTHR
5   THRILEASNPROARGPHEGLNGLUALAASP
6   SERPROTHRLEU

Samples:

pH_6.1: D2O, [U-2H], 5%; sodium phosphate 50 mM; sodium chloride 100 mM; DTT 1 mM; DSS 0.1 mM; beta7 integrin, [U-15N], 0.05 mM; H2O 95%

pH_4.5: D2O, [U-2H], 5%; sodium acetate 20 mM; sodium azide 0.02%; DSS 0.1 mM; beta7 integrin, [U-15N], 0.2 mM; H2O 95%

pH6.1: ionic strength: 200 mM; pH: 6.1; pressure: 1 atm; temperature: 298 K

pH4.5: ionic strength: 10 mM; pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCpH_6.1isotropicpH6.1
3D 1H-15N NOESYpH_4.5isotropicpH4.5
3D 1H-15N TOCSYpH_4.5isotropicpH4.5
2D 1H-15N HSQCpH_4.5isotropicpH4.5

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

Omega Spectrometer Operating Software vBeta 6.0.3b2, GE - collection

ANALYSIS, Boucher and Stevens - chemical shift assignment

NMR spectrometers:

  • GE OMEGA 600 MHz
  • GE OMEGA 950 MHz

Related Database Links:

PDB
DBJ BAG10580 BAH12742
GB AAA36118 AAA59184 AAA59185 AAB21332 AAB23688
REF NP_000880 XP_001087864 XP_001141622 XP_003831078 XP_004053261
SP P26010
AlphaFold P26010

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks