BMRB Entry 16236

Name: Bruno RRM3+
Published: 2009-11-23

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PDB ID: 2khc
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16236
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Bruno RRM3+

Deposition date:

2009-04-01

Original release date:

2009-11-23

Authors:

Lyon, Angeline; Reveal, Brad; Macdonald, Paul; Hoffman, David

Citation:

Citation: Lyon, Angeline; Reveal, Brad; Macdonald, Paul; Hoffman, David. "Bruno protein contains an expanded RNA recognition motif" Biochemistry 48, 12202-12212 (2009).
PubMed: 19919093

Assembly members:

Assembly members:
Bruno_RRM3+, polymer, 118 residues, 12296.124 Da.

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Bruno_RRM3+: AAGLPQFGSASALSTSPLAS VALSAAAAAAAGKQIEGPEG CNLFIYHLPQEFTDTDLAST FLPFGNVISAKVFIDKQTSL SKCFGFVSFDNPDSAQVAIK AMNGFQVGTKRLKVQLKK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	434
15N chemical shifts	117
1H chemical shifts	782

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Bruno_RRM3+	1

Entities:

Entity 1, Bruno_RRM3+ 118 residues - 12296.124 Da.

1

ALA

GLY

LEU

PRO

GLN

PHE

GLY

SER

ALA

2

SER

ALA

LEU

SER

THR

SER

PRO

LEU

ALA

SER

3

VAL

ALA

LEU

SER

ALA

4

ALA

GLY

LYS

GLN

ILE

GLU

GLY

PRO

GLU

GLY

5

CYS

ASN

LEU

PHE

ILE

TYR

HIS

LEU

PRO

GLN

6

GLU

PHE

THR

ASP

THR

ASP

LEU

ALA

SER

THR

7

PHE

LEU

PRO

PHE

GLY

ASN

VAL

ILE

SER

ALA

8

LYS

VAL

PHE

ILE

ASP

LYS

GLN

THR

SER

LEU

9

SER

LYS

CYS

PHE

GLY

PHE

VAL

SER

PHE

ASP

10

ASN

PRO

ASP

SER

ALA

GLN

VAL

ALA

ILE

LYS

11

ALA

MET

ASN

GLY

PHE

GLN

VAL

GLY

THR

LYS

12

ARG

LEU

LYS

VAL

GLN

LEU

LYS

Samples:

Bruno_RRM3+_1: 1,0 mM Bruno RRM3+, [U-100% 13C; U-100% 15N], 1 mM; potassium phosphate 1 mM; sodium chloride300 – 400 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 260 mM; pH: 6; pressure: 1.0 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-13C NOESY	Bruno_RRM3+_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	Bruno_RRM3+_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	Bruno_RRM3+_1	isotropic	sample_conditions_1

Software:

CNS v1.1., Brunger, Adams, Clore, Gros, Nilges and Read - simulated annealing

NMR spectrometers:

Varian Inova 500 MHz

PDB	2KHC
GB	AAL49272 AAM50707 AAN10813 ACL86931 AET11743
REF	NP_723740 XP_002015057