BMRB Entry 16231

Title:
Solution Structure of a Pathogen Recognition Domain from a Lepidopteran Insect, Plodia interpunctella
Deposition date:
2009-03-29
Original release date:
2012-08-06
Authors:
Dai, Huaien; Hiromasa, Yasuaki; Fabrick, Jeffrey; VanderVelde, David; Kanost, Michael; Krishnamoorthi, Ramaswamy
Citation:

Citation: Dai, Huaien; Hiromasa, Yasuaki; An, Chunju; Fabrick, Jeffrey; VanderVelde, David; Kanost, Michael; Krishnamoorthi, Ramaswamy. "Solution Structure of a Pathogen Recognition Domain from a Lepidopteran Insect, Plodia interpunctella"  .

Assembly members:

Assembly members:
Pathogen Recognition Domain, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Indianmeal moth   Taxonomy ID: 58824   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Plodia interpunctella

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pPROEX-HTb

Experimental source:

Natural source:   Common Name: Indianmeal moth   Taxonomy ID: 58824   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Plodia interpunctella

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pPROEX-HTb

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts415
15N chemical shifts110
1H chemical shifts700

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pathogen Recognition Domain1

Entities:

Entity 1, Pathogen Recognition Domain 113 residues - Formula weight is not available

1   GLNTYRVALVALPROSERALALYSLEUGLU
2   ALAILETYRPROARGGLYLEUARGVALSER
3   ILEPROASPASPGLYPHESERLEUPHEALA
4   PHEHISGLYLYSLEUASNGLUGLUMETASP
5   GLYLEUGLUALAGLYHISTRPALAARGASP
6   ILETHRLYSPROLYSGLUGLYARGTRPTHR
7   PHEARGASPARGASNALALYSLEULYSLEU
8   GLYASPLYSILETYRPHETRPTHRTYRVAL
9   ILELYSASPGLYLEUGLYTYRARGGLNASP
10   ASNGLYGLUTRPTHRVALTHRGLUPHEVAL
11   ASNGLUASPGLYTHRPROALAASPTHRSER
12   LEUGLUPRO

Samples:

sample_1: entity_2, [U-100% 13C; U-100% 15N], 1.5-2.0 mM; sodium phosphate buffer 20 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAM95970
SP Q8MU95
AlphaFold Q8MU95

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks