BMRB Entry 16229

Name: 1H, 13C, and 15N Chemical Shift Assignments for ring1B C-terminal domain/ cbx7 CBOX complex
Published: 2010-12-09

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16229

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N Chemical Shift Assignments for ring1B C-terminal domain/ cbx7 CBOX complex

Deposition date:

2009-03-27

Original release date:

2010-12-09

Authors:

Wang, Renjing; Kim, Chongwoo A.; Ilangovan, Udayar

Citation:

Citation: Wang, Renjing; Taylor, Alexander; Leal, Belinda; Chadwell, Linda; Ilangovan, Udayar; Robinson, Angela; Schirf, Virgil; Hart, P. John; Lafer, Eileen; Demeler, Borries; Hinck, Andrew; McEwen, Donald; Kim, Chongwoo. "Polycomb group targeting through different binding partners of RING1B C-terminal domain." Structure 18, 966-975 (2010).
PubMed: 20696397

Assembly members:

Assembly members:
C-RING1B, polymer, 123 residues, Formula weight is not available
CBX7_Cbox, polymer, 56 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3c and pET30a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-RING1B: MEKTRGASEIELVFRPHPTL MEKDDSAQTRYIKTSGNATV DHLSKYLAVRLALEELRSKG ESNQMNLDTASEKQYTIYIA TASGQFTVLNGSFSLELVSE KYWKVNKPMELYYAPTKEHK SRD
CBX7_Cbox: MEKTRESWTPALPSSEVTVT DITANSITVTFREAQAAEGF FRDRSGKFSRHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	157
15N chemical shifts	39
1H chemical shifts	225

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ring1b C-terminal domain	1
2	cbx7 CBOX	2

Entities:

Entity 1, ring1b C-terminal domain 123 residues - Formula weight is not available

1

MET

GLU

LYS

THR

ARG

GLY

ALA

SER

GLU

ILE

2

GLU

LEU

VAL

PHE

ARG

PRO

HIS

PRO

THR

LEU

3

MET

GLU

LYS

ASP

SER

ALA

GLN

THR

ARG

4

TYR

ILE

LYS

THR

SER

GLY

ASN

ALA

THR

VAL

5

ASP

HIS

LEU

SER

LYS

TYR

LEU

ALA

VAL

ARG

6

LEU

ALA

LEU

GLU

LEU

ARG

SER

LYS

GLY

7

GLU

SER

ASN

GLN

MET

ASN

LEU

ASP

THR

ALA

8

SER

GLU

LYS

GLN

TYR

THR

ILE

TYR

ILE

ALA

9

THR

ALA

SER

GLY

GLN

PHE

THR

VAL

LEU

ASN

10

GLY

SER

PHE

SER

LEU

GLU

LEU

VAL

SER

GLU

11

LYS

TYR

TRP

LYS

VAL

ASN

LYS

PRO

MET

GLU

12

LEU

TYR

ALA

PRO

THR

LYS

GLU

HIS

LYS

13

SER

ARG

ASP

Entity 2, cbx7 CBOX 56 residues - Formula weight is not available

1

MET

GLU

LYS

THR

ARG

GLU

SER

TRP

THR

PRO

2

ALA

LEU

PRO

SER

GLU

VAL

THR

VAL

THR

3

ASP

ILE

THR

ALA

ASN

SER

ILE

THR

VAL

THR

4

PHE

ARG

GLU

ALA

GLN

ALA

GLU

GLY

PHE

5

PHE

ARG

ASP

ARG

SER

GLY

LYS

PHE

SER

ARG

6

HIS

Samples:

15N: C-RING1B, [U-99% 15N], 1.0 mM; CBX7 Cbox Complex, [U-99% 15N], 1.0 mM; H20, Natural Abundance, 95%; D20, Natural Abundance, 5%

15N_13C: C-RING1B, [U-99% 13C; U-99% 15N], 1.0 mM; CBX7 Cbox Complex, [U-99% 13C; U-99% 15N], 1.0 mM; H20, Natural Abundance, 95%; D20, Natural Abundance, 5%

sample_conditions_1: pH: 6.0; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	15N	isotropic	sample_conditions_1
2D 1H-13C HSQC	15N_13C	isotropic	sample_conditions_1
3D CBCA(CO)NH	15N_13C	isotropic	sample_conditions_1
3D C(CO)NH	15N_13C	isotropic	sample_conditions_1
3D HNCA	15N_13C	isotropic	sample_conditions_1
3D HNCACB	15N_13C	isotropic	sample_conditions_1
3D HCCH-TOCSY	15N_13C	isotropic	sample_conditions_1
3D HNCO	15N_13C	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

xwinnmr, Bruker Biospin - collection

TOPSPIN, Bruker Biospin - collection

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

Bruker Avance 700 MHz
Bruker DRX 600 MHz

PDB	3GS2 3H8H 3IXS 3GS2
DBJ	BAB28186 BAB28663 BAC26898 BAC41075 BAE26638 BAI46775
EMBL	CAA71596 CAA73321 CAA73380 CAH91565 CAJ18505
GB	AAD29717 AAH12583 AAH20122 AAH98941 AAI48045 AAH51773 AAY68391 ABZ92314 AIC56058 EAW60298
REF	NP_001020838 NP_001094673 NP_001127433 NP_009143 NP_035407 NP_001179547 NP_001247702 NP_783640 XP_002743799 XP_002914600
SP	Q4KLY4 Q5R9J5 Q99496 Q9CQJ4 O95931
TPG	DAA20975 DAA29144
AlphaFold	Q4KLY4 Q5R9J5 Q99496 Q9CQJ4 O95931