BMRB Entry 16229

Title:
1H, 13C, and 15N Chemical Shift Assignments for ring1B C-terminal domain/ cbx7 CBOX complex
Deposition date:
2009-03-27
Original release date:
2010-12-09
Authors:
Wang, Renjing; Kim, Chongwoo A.; Ilangovan, Udayar
Citation:

Citation: Wang, Renjing; Taylor, Alexander; Leal, Belinda; Chadwell, Linda; Ilangovan, Udayar; Robinson, Angela; Schirf, Virgil; Hart, P. John; Lafer, Eileen; Demeler, Borries; Hinck, Andrew; McEwen, Donald; Kim, Chongwoo. "Polycomb group targeting through different binding partners of RING1B C-terminal domain."  Structure 18, 966-975 (2010).
PubMed: 20696397

Assembly members:

Assembly members:
C-RING1B, polymer, 123 residues, Formula weight is not available
CBX7_Cbox, polymer, 56 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3c and pET30a

Data sets:
Data typeCount
13C chemical shifts157
15N chemical shifts39
1H chemical shifts225

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ring1b C-terminal domain1
2cbx7 CBOX2

Entities:

Entity 1, ring1b C-terminal domain 123 residues - Formula weight is not available

1   METGLULYSTHRARGGLYALASERGLUILE
2   GLULEUVALPHEARGPROHISPROTHRLEU
3   METGLULYSASPASPSERALAGLNTHRARG
4   TYRILELYSTHRSERGLYASNALATHRVAL
5   ASPHISLEUSERLYSTYRLEUALAVALARG
6   LEUALALEUGLUGLULEUARGSERLYSGLY
7   GLUSERASNGLNMETASNLEUASPTHRALA
8   SERGLULYSGLNTYRTHRILETYRILEALA
9   THRALASERGLYGLNPHETHRVALLEUASN
10   GLYSERPHESERLEUGLULEUVALSERGLU
11   LYSTYRTRPLYSVALASNLYSPROMETGLU
12   LEUTYRTYRALAPROTHRLYSGLUHISLYS
13   SERARGASP

Entity 2, cbx7 CBOX 56 residues - Formula weight is not available

1   METGLULYSTHRARGGLUSERTRPTHRPRO
2   ALALEUPROSERSERGLUVALTHRVALTHR
3   ASPILETHRALAASNSERILETHRVALTHR
4   PHEARGGLUALAGLNALAALAGLUGLYPHE
5   PHEARGASPARGSERGLYLYSPHESERARG
6   HISHISHISHISHISHIS

Samples:

15N: C-RING1B, [U-99% 15N], 1.0 mM; CBX7 Cbox Complex, [U-99% 15N], 1.0 mM; H20, Natural Abundance, 95%; D20, Natural Abundance, 5%

15N_13C: C-RING1B, [U-99% 13C; U-99% 15N], 1.0 mM; CBX7 Cbox Complex, [U-99% 13C; U-99% 15N], 1.0 mM; H20, Natural Abundance, 95%; D20, Natural Abundance, 5%

sample_conditions_1: pH: 6.0; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15Nisotropicsample_conditions_1
2D 1H-13C HSQC15N_13Cisotropicsample_conditions_1
3D CBCA(CO)NH15N_13Cisotropicsample_conditions_1
3D C(CO)NH15N_13Cisotropicsample_conditions_1
3D HNCA15N_13Cisotropicsample_conditions_1
3D HNCACB15N_13Cisotropicsample_conditions_1
3D HCCH-TOCSY15N_13Cisotropicsample_conditions_1
3D HNCO15N_13Cisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

xwinnmr, Bruker Biospin - collection

TOPSPIN, Bruker Biospin - collection

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAB28186 BAB28663 BAC26898 BAC41075 BAE26638 BAI46775
EMBL CAA71596 CAA73321 CAA73380 CAH91565 CAJ18505
GB AAD29717 AAH12583 AAH20122 AAH98941 AAI48045 AAH51773 AAY68391 ABZ92314 AIC56058 EAW60298
REF NP_001020838 NP_001094673 NP_001127433 NP_009143 NP_035407 NP_001179547 NP_001247702 NP_783640 XP_002743799 XP_002914600
SP Q4KLY4 Q5R9J5 Q99496 Q9CQJ4 O95931
TPG DAA20975 DAA29144
AlphaFold Q4KLY4 Q5R9J5 Q99496 Q9CQJ4 O95931

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks