BMRB Entry 16198
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16198
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Citation: Thakur, Anushikha; Balasubramanyam, Chittoor; Silva, Patrick; Atreya, Hanudatta. "1H.13C and 15N backbone and side-chain resonance assignments of N-terminal domain of a mitochondrial inner membrane translocase (TIM23) from S. cerevisiae" .
Assembly members:
Tim23, polymer, 104 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3a
Entity Sequences (FASTA):
Tim23: MSWLFGDKTPTDDANAAVGG
QDTTKPKELSLKQSLGFEPN
INNIISGPGGMHVDTARLHP
LAGLDKGVEYLDLEEEQLSS
LEGSQGLIPSRGWTDDLCHH
HHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 359 |
| 15N chemical shifts | 90 |
| 1H chemical shifts | 555 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tim23 | 1 |
Entities:
Entity 1, Tim23 104 residues - Formula weight is not available
| 1 | MET | SER | TRP | LEU | PHE | GLY | ASP | LYS | THR | PRO | ||||
| 2 | THR | ASP | ASP | ALA | ASN | ALA | ALA | VAL | GLY | GLY | ||||
| 3 | GLN | ASP | THR | THR | LYS | PRO | LYS | GLU | LEU | SER | ||||
| 4 | LEU | LYS | GLN | SER | LEU | GLY | PHE | GLU | PRO | ASN | ||||
| 5 | ILE | ASN | ASN | ILE | ILE | SER | GLY | PRO | GLY | GLY | ||||
| 6 | MET | HIS | VAL | ASP | THR | ALA | ARG | LEU | HIS | PRO | ||||
| 7 | LEU | ALA | GLY | LEU | ASP | LYS | GLY | VAL | GLU | TYR | ||||
| 8 | LEU | ASP | LEU | GLU | GLU | GLU | GLN | LEU | SER | SER | ||||
| 9 | LEU | GLU | GLY | SER | GLN | GLY | LEU | ILE | PRO | SER | ||||
| 10 | ARG | GLY | TRP | THR | ASP | ASP | LEU | CYS | HIS | HIS | ||||
| 11 | HIS | HIS | HIS | HIS |
Samples:
sample_1: Tim23, [U-100% 13C; U-100% 15N], 1 mM; potassium phosphate 50 mM; sodium chloride 50 mM; D2O 10%; H2O 90%
sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
XEASY, Bartels et al. - data analysis
NMR spectrometers:
- Bruker Avance 700 MHz
Related Database Links:
| DBJ | GAA26110 |
| EMBL | CAA50640 CAA52274 CAA96296 CAY82210 |
| GB | AHY77105 AJP41342 AJT01777 AJT02149 AJT02522 |
| REF | NP_014414 |
| SP | P32897 |
| TPG | DAA10558 |
| AlphaFold | P32897 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks