BMRB Entry 16192

Name: NMR structure of the first qRRM of hnRNP F in complex with AGGGAU G-tract RNA
Published: 2010-06-15

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16192

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of the first qRRM of hnRNP F in complex with AGGGAU G-tract RNA

Deposition date:

2009-03-02

Original release date:

2010-06-15

Authors:

Allain, Frederic; Dominguez, Cyril

Citation:

Citation: Dominguez, Cyril; Fisette, Jean-Francois; Chabot, Benoit; Allain, Frederic H-T. "Structural basis of G-tract recognition and encaging by hnRNP F quasi-RRMs." Nat. Struct. Mol. Biol. 17, 853-861 (2010).
PubMed: 20526337

Assembly members:

Assembly members:
entity_1, polymer, 6 residues, 1955.251 Da.
first qRRM of hnRNP F, polymer, 102 residues, 11475.048 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AGGGAU
first qRRM of hnRNP F: MMLGPEGGEGFVVKLRGLPW SCSVEDVQNFLSDCTIHDGA AGVHFIYTREGRQSGEAFVE LGSEDDVKMALKKDRESMGH RYIEVFKSHRTEMDWVLKHS GP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	258
15N chemical shifts	102
1H chemical shifts	685

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AGGGAU G-tract RNA	1
2	first qRRM of hnRNP F	2

Entities:

Entity 1, AGGGAU G-tract RNA 6 residues - 1955.251 Da.

1

A

G

A

U

Entity 2, first qRRM of hnRNP F 102 residues - 11475.048 Da.

1

MET

LEU

GLY

PRO

GLU

GLY

GLU

GLY

2

PHE

VAL

LYS

LEU

ARG

GLY

LEU

PRO

TRP

3

SER

CYS

SER

VAL

GLU

ASP

VAL

GLN

ASN

PHE

4

LEU

SER

ASP

CYS

THR

ILE

HIS

ASP

GLY

ALA

5

ALA

GLY

VAL

HIS

PHE

ILE

TYR

THR

ARG

GLU

6

GLY

ARG

GLN

SER

GLY

GLU

ALA

PHE

VAL

GLU

7

LEU

GLY

SER

GLU

ASP

VAL

LYS

MET

ALA

8

LEU

LYS

ASP

ARG

GLU

SER

MET

GLY

HIS

9

ARG

TYR

ILE

GLU

VAL

PHE

LYS

SER

HIS

ARG

10

THR

GLU

MET

ASP

TRP

VAL

LEU

LYS

HIS

SER

11

GLY

PRO

Samples:

sample_1: entity_11 – 1.5 mM; entity_2, [U-13C; U-15N], 1 – 1.5 mM; NACL 50 mM; Na2HPO4 20 mM

sample_conditions_1: ionic strength: 70 mM; pH: 7.0; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY v3.113, Goddard - chemical shift assignment

CYANA v2.1, Herrmann, Guntert and Wuthrich - structure solution

AMBER v7.0, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 900 MHz

SWS	.
PDB	2HGL 2KFY