BMRB Entry 16182

Title:
The solution conformation of the leader sequence of the prepropeptide for the microcin-like patellamides
Deposition date:
2009-02-22
Original release date:
2010-10-18
Authors:
Jaspars, Marcel; Wright, Stephen; Houssen, Wael; Kelly, Sharon; Kalverda, Arnout
Citation:

Citation: Houssen, Wael; Wright, Stephen; Kalverda, Arnout; Thompson, Gary; Kelly, Sharon; Jaspars, Marcel. "Solution structure of the leader sequence of the patellamide precursor peptide, PatE1-34."  Chembiochem 11, 1867-1873 (2010).
PubMed: 20715266

Assembly members:

Assembly members:
Leader peptide, polymer, 35 residues, 3707.293 Da.

Natural source:

Natural source:   Common Name: cyanobacteria   Taxonomy ID: 1216   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Prochloron not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Leader peptide: MNKKNILPQQGQPVIRLTAG QLSSQLAELSEEAX

Data sets:
Data typeCount
1H chemical shifts255

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PatE prepropeptide1

Entities:

Entity 1, PatE prepropeptide 35 residues - 3707.293 Da.

1   METASNLYSLYSASNILELEUPROGLNGLN
2   GLYGLNPROVALILEARGLEUTHRALAGLY
3   GLNLEUSERSERGLNLEUALAGLULEUSER
4   GLUGLUALANLW

Samples:

sample_1: entity 1.0 mM; H2O 50%; TFE, [U-99% 2H], 50%; disodium hydrogen phosphate 5 mM; sodium dihydrogen phosphate 5 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 65 mM; pH: 5.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
1D 1Hsample_1isotropicsample_conditions_1

Software:

X-PLOR v3.851, Brunger - restrained molecular dynamics calculations

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

GB AY986476