BMRB Entry 16178
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16178
MolProbity Validation Chart
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NMR-STAR v3 text file.
XML gzip file.
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Citation: Gao, Bing; Xu, Jia; Rodriguezc, M. del; Lanz-Mendozac, Humberto; Hernandez-Rivas, Rosaura; Du, Weihong; Zhu, Shunyi. "Characterization of two linear cationic antimalarial peptides in the scorpion Mesobuthus eupeus" Biochimie 92, 350-359 (2010).
PubMed: 20097251
Assembly members:
MeVamp-4, polymer, 24 residues, 2761.265 Da.
Natural source: Common Name: lesser Asian scorpion Taxonomy ID: 34648 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mesobuthus eupeus
Experimental source: Production method: chemical synthesis Host organism: Mesobuthus eupeus
Entity Sequences (FASTA):
MeVamp-4: GRGREFMSNLKEKLSGVKEK
MKNS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 170 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MeVamp-4 | 1 |
Entities:
Entity 1, MeVamp-4 24 residues - 2761.265 Da.
| 1 | GLY | ARG | GLY | ARG | GLU | PHE | MET | SER | ASN | LEU | ||||
| 2 | LYS | GLU | LYS | LEU | SER | GLY | VAL | LYS | GLU | LYS | ||||
| 3 | MET | LYS | ASN | SER |
Samples:
sample_1: MeVamp-4 1 mM; d3-trifluoroethanol 50%; H2O 50%
sample_conditions_1: pH: 2.7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2.1, Bruker Biospin - collection, processing
SPARKY v3.114, Goddard - chemical shift assignment, peak picking
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation
NMR spectrometers:
- Bruker Avance 600 MHz