BMRB Entry 16168

Name: STRUCTURE OF THE NA,K-ATPASE REGULATORY PROTEIN FXYD1 IN MICELLES
Published: 2009-03-06

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PDB ID: 2jo1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16168
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

STRUCTURE OF THE NA,K-ATPASE REGULATORY PROTEIN FXYD1 IN MICELLES

Deposition date:

2009-02-11

Original release date:

2009-03-06

Authors:

Teriete, P.; Franzin, C.; Choi, J.; Marassi, F.

Citation:

Citation: Teriete, P.; Franzin, C.; Choi, J.; Marassi, F.. "Structure of the Na,K-ATPase regulatory protein FXYD1 in micelles." Biochemistry 46, 6774-6783 (2007).
PubMed: 17511473

Assembly members:

Assembly members:
FXYD1, polymer, 72 residues, 8396.635 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI Vector: PBCL173

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FXYD1: ESPKEHDPFTYDYQSLQIGG LVIAGILFILGILIVLSRRC RCKFNQQQRTGEPDEEEGTF RSSIRRLSTRRR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	138
15N chemical shifts	67
1H chemical shifts	145

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FXYD1	1

Entities:

Entity 1, FXYD1 72 residues - 8396.635 Da.

1

GLU

SER

PRO

LYS

GLU

HIS

ASP

PRO

PHE

THR

2

TYR

ASP

TYR

GLN

SER

LEU

GLN

ILE

GLY

3

LEU

VAL

ILE

ALA

GLY

ILE

LEU

PHE

ILE

LEU

4

GLY

ILE

LEU

ILE

VAL

LEU

SER

ARG

CYS

5

ARG

CYS

LYS

PHE

ASN

GLN

ARG

THR

6

GLY

GLU

PRO

ASP

GLU

GLY

THR

PHE

7

ARG

SER

ILE

ARG

LEU

SER

THR

ARG

8

ARG

Samples:

sample_1: FXYD1, [U-100% 13C; U-100% 15N], 0.5 mM; sodium citrate 20 mM; DTT 10 mM; SDS 500 mM; H2O 90%; D2O 10%

sample_2: FXYD1, [U-100% 15N], 1 mM; sodium citrate 20 mM; DTT 10 mM; SDS 500 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 60 mM; pH: 5.0; pressure: 1 atm; temperature: 313.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

X-PLOR NIH, C.D.SCHWIETERS,J.J.KUSZEWSKI,N.TJ - refinement

SPARKY v3.11, Goddard - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

BRUKER DMX 600 MHz
BRUKER DMX 500 MHz

PDB	2JO1
DBJ	BAF82500 BAG73170
GB	AAC51286 AAH32800 ABW03332 ABW03689 ACT90639
REF	NP_001265646 NP_001265647 NP_005022 NP_068702 XP_002829099
SP	O00168
AlphaFold	O00168