BMRB Entry 16168

Title:
STRUCTURE OF THE NA,K-ATPASE REGULATORY PROTEIN FXYD1 IN MICELLES   PubMed: 17511473
Deposition date:
2009-02-11
Original release date:
2009-03-06
Authors:
Teriete, P.; Franzin, C.; Choi, J.; Marassi, F.
Citation:

Citation: Teriete, P.; Franzin, C.; Choi, J.; Marassi, F.. "Structure of the Na,K-ATPase regulatory protein FXYD1 in micelles."  Biochemistry 46, 6774-6783 (2007).

Assembly members:

Assembly members:
FXYD1, polymer, 72 residues, 8396.635 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: ESCHERICHIA COLI   Vector: PBCL173

Experimental source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: ESCHERICHIA COLI   Vector: PBCL173

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts138
15N chemical shifts67
1H chemical shifts145

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FXYD11

Entities:

Entity 1, FXYD1 72 residues - 8396.635 Da.

1   GLUSERPROLYSGLUHISASPPROPHETHR
2   TYRASPTYRGLNSERLEUGLNILEGLYGLY
3   LEUVALILEALAGLYILELEUPHEILELEU
4   GLYILELEUILEVALLEUSERARGARGCYS
5   ARGCYSLYSPHEASNGLNGLNGLNARGTHR
6   GLYGLUPROASPGLUGLUGLUGLYTHRPHE
7   ARGSERSERILEARGARGLEUSERTHRARG
8   ARGARG

Samples:

sample_1: FXYD1, [U-100% 13C; U-100% 15N], 0.5 mM; sodium citrate 20 mM; DTT 10 mM; SDS 500 mM; H2O 90%; D2O 10%

sample_2: FXYD1, [U-100% 15N], 1 mM; sodium citrate 20 mM; DTT 10 mM; SDS 500 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 60 mM; pH: 5.0; pressure: 1 atm; temperature: 313.15 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, C.D.SCHWIETERS,J.J.KUSZEWSKI,N.TJ - refinement

SPARKY v3.11, Goddard - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

  • BRUKER DMX 600 MHz
  • BRUKER DMX 500 MHz

Related Database Links:

PDB
DBJ BAF82500 BAG73170
GB AAC51286 AAH32800 ABW03332 ABW03689 ACT90639
REF NP_001265646 NP_001265647 NP_005022 NP_068702 XP_002829099
SP O00168
AlphaFold O00168

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts