BMRB Entry 16159

Name: Beta1A Integrin Cytoplasmic Tail 1H and 15N Chemical Shift Assignments
Published: 2009-10-07

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16159

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Beta1A Integrin Cytoplasmic Tail 1H and 15N Chemical Shift Assignments

Deposition date:

2009-02-03

Original release date:

2009-10-07

Authors:

Anthis, Nicholas; Wegener, Kate; Campbell, Iain

Citation:

Citation: Anthis, Nicholas; Wegener, Kate; Ye, Feng; Kim, Chungho; Goult, Benjamin; Lowe, Edward; Vakonakis, Ioannis; Bate, Neil; Critchley, David; Ginsberg, Mark; Campbell, Iain. "The structure of an integrin/talin complex reveals the basis of inside-out signal transduction." EMBO J. 28, 3623-3632 (2009).
PubMed: 19798053

Assembly members:

Assembly members:
beta1A_integrin, polymer, 49 residues, 5710 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta1A_integrin: GPKLLMIIHDRREFAKFEKE KMNAKWDTGENPIYKSAVTT VVNPKYEGK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2

Data type	Count
15N chemical shifts	100
1H chemical shifts	347

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	beta1A integrin tail	1

Entities:

Entity 1, beta1A integrin tail 49 residues - 5710 Da.

This is the cytoplasmic tail of the beta1A integrin tail. The first two residues (GP) are a cloning artifact.

1

GLY

PRO

LYS

LEU

MET

ILE

HIS

ASP

2

ARG

GLU

PHE

ALA

LYS

PHE

GLU

LYS

GLU

3

LYS

MET

ASN

ALA

LYS

TRP

ASP

THR

GLY

GLU

4

ASN

PRO

ILE

TYR

LYS

SER

ALA

VAL

THR

5

VAL

ASN

PRO

LYS

TYR

GLU

GLY

LYS

Samples:

pH_6.1: D2O, [U-2H], 5%; sodium phosphate 50 mM; sodium chloride 100 mM; DTT 1 mM; DSS 0.1 mM; beta1A integrin, [U-15N], 0.05 mM

pH_5.0: D2O, [U-2H], 5%; sodium acetate 20 mM; sodium azide 0.02%; DSS 0.1 mM; beta1A integrin, [U-15N], 1 mM

sample_conditions_1: pH: 6.1; pressure: 1.0 atm; temperature: 298 K

sample_conditions_2: pH: 5.0; pressure: 1.0 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	pH_6.1	isotropic	sample_conditions_1
3D 1H-15N NOESY	pH_5.0	isotropic	sample_conditions_2
3D 1H-15N TOCSY	pH_5.0	isotropic	sample_conditions_2
2D 1H-15N HSQC	pH_5.0	isotropic	sample_conditions_2

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

Omega Spectrometer Operating Software vBeta 6.0.3b2, GE - collection

ANALYSIS, Boucher and Stevens - chemical shift assignment

NMR spectrometers:

GE OMEGA 600 MHz

UNP	P05556
DBJ	BAC39546 BAC40532 BAE39731 BAF84386 BAG72587
EMBL	CAA30790 CAA33272 CAA68738 CAD97649 CAF97757
GB	AAA48926 AAA49889 AAA49890 AAA59182 AAA74402
PIR	PH0104
REF	NP_001030143 NP_001030159 NP_001034343 NP_001041625 NP_001081286
SP	A5Z1X6 B0FYY4 P05556 P07228 P09055
TPG	DAA23553
AlphaFold	Q8WUM6 A5Z1X6 B0FYY4 P05556 P07228 P09055