BMRB Entry 16158

Title:
Beta1D Integrin Cytoplasmic Tail 1H and 15N Chemical Shift Assignments
Deposition date:
2009-02-03
Original release date:
2009-10-07
Authors:
Anthis, Nicholas; Wegener, Kate; Campbell, Iain
Citation:

Citation: Anthis, Nicholas; Wegener, Kate; Ye, Feng; Kim, Chungho; Goult, Benjamin; Lowe, Edward; Vakonakis, Ioannis; Bate, Neil; Critchley, David; Ginsberg, Mark; Campbell, Iain. "The structure of an integrin/talin complex reveals the basis of inside-out signal transduction."  EMBO J. 28, 3623-3632 (2009).
PubMed: 19798053

Assembly members:

Assembly members:
beta1D_integrin, polymer, 52 residues, 6178 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta1D_integrin: GPKLLMIIHDRREFAKFEKE KMNAKWDTQENPIYKSPINN FKNPNYGRKAGL

Data sets:
Data typeCount
15N chemical shifts55
1H chemical shifts62

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1beta1D integrin tail1

Entities:

Entity 1, beta1D integrin tail 52 residues - 6178 Da.

This is the cytoplasmic tail of the beta1D integrin tail. The first two residues (GP) are a cloning artifact.

1   GLYPROLYSLEULEUMETILEILEHISASP
2   ARGARGGLUPHEALALYSPHEGLULYSGLU
3   LYSMETASNALALYSTRPASPTHRGLNGLU
4   ASNPROILETYRLYSSERPROILEASNASN
5   PHELYSASNPROASNTYRGLYARGLYSALA
6   GLYLEU

Samples:

pH_6.1: D2O, [U 2H], 5%; sodium phosphate 50 mM; sodium chloride 100 mM; DTT 1 mM; DSS 0.1 mM; beta1D integrin, [U-15N], 0.05 mM

sample_conditions_1: pH: 6.1; pressure: 1.0 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCpH_6.1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

Omega Spectrometer Operating Software vBeta 6.0.3b2, GE - collection

ANALYSIS, Boucher and Stevens - chemical shift assignment

NMR spectrometers:

  • GE OMEGA 500 MHz

Related Database Links:

BMRB 16162
PDB
GB AAA59182 AAA79833 AAA80243 AAA81366 AAA88821
PIR PH0104 S66396
REF NP_001288146 NP_391988 XP_003364388 XP_004387928 XP_004401881

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks