BMRB Entry 16137

Name: NMR structure of the first SAM domain from AIDA1
Published: 2009-10-20

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PDB ID: 2ke7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16137
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of the first SAM domain from AIDA1

Deposition date:

2009-01-27

Original release date:

2009-10-20

Authors:

Donaldson, Logan

Citation:

Citation: Donaldson, Logan. "NMR structure of the first SAM domain from AIDA1" .

Assembly members:

Assembly members:
AIDA1_SAM1, polymer, 103 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pDEST586

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AIDA1_SAM1: GSDITSLYKKAGSEMNGPRC PVQTVGQWLESIGLPQYENH LMANGFDNVQFMGSNVMEDQ DLLEIGILNSGHRQRILQAI QLLPKMRPIGHDGYHPTSVA EWL

Data sets:

assigned_chemical_shifts
- assignments_aida3

Data type	Count
13C chemical shifts	257
15N chemical shifts	61
1H chemical shifts	411

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AIDA1_SAM1	1

Entities:

Entity 1, AIDA1_SAM1 103 residues - Formula weight is not available

1

GLY

SER

ASP

ILE

THR

SER

LEU

TYR

LYS

2

ALA

GLY

SER

GLU

MET

ASN

GLY

PRO

ARG

CYS

3

PRO

VAL

GLN

THR

VAL

GLY

GLN

TRP

LEU

GLU

4

SER

ILE

GLY

LEU

PRO

GLN

TYR

GLU

ASN

HIS

5

LEU

MET

ALA

ASN

GLY

PHE

ASP

ASN

VAL

GLN

6

PHE

MET

GLY

SER

ASN

VAL

MET

GLU

ASP

GLN

7

ASP

LEU

GLU

ILE

GLY

ILE

LEU

ASN

SER

8

GLY

HIS

ARG

GLN

ARG

ILE

LEU

GLN

ALA

ILE

9

GLN

LEU

PRO

LYS

MET

ARG

PRO

ILE

GLY

10

HIS

ASP

GLY

TYR

HIS

PRO

THR

SER

VAL

ALA

11

GLU

TRP

LEU

Samples:

NMRsample: AIDA1 SAM1, [U-98% 13C; U-98% 15N], 0.8 mM; D2O 10%; H2O 90%; sodium chloride 150 mM; sodium phosphate 20 mM; sodium azide 0.05%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.8; pressure: 1.0 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	NMRsample	isotropic	sample_conditions_1
2D 1H-13C HSQC	NMRsample	isotropic	sample_conditions_1
3D CBCA(CO)NH	NMRsample	isotropic	sample_conditions_1
3D C(CO)NH	NMRsample	isotropic	sample_conditions_1
3D HNCO	NMRsample	isotropic	sample_conditions_1
3D HNCACB	NMRsample	isotropic	sample_conditions_1
3D H(CCO)NH	NMRsample	isotropic	sample_conditions_1
3D 1H-15N NOESY	NMRsample	isotropic	sample_conditions_1
3D 1H-13C NOESY	NMRsample	isotropic	sample_conditions_1

Software:

X-PLOR NIH v2.21, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMRView v5, Johnson, One Moon Scientific - peak picking

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

Varian NMRS 600 MHz

GB	AY283057.2 AAF80756 AAH26313 AAH68451 AAH98373 AAI49112
PDB	2KE7
DBJ	BAE01854 BAG57507 BAI47262
EMBL	CAH91693
REF	NP_001095761 NP_001121558 NP_001125990 NP_001170868 NP_001190995
TPG	DAA29568