BMRB Entry 16127

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16127
MolProbity Validation Chart

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Title:
Solution Structure of the SAP30 zinc finger motif
Deposition date:
2009-01-21
Original release date:
2009-02-23
Authors:
Radhakrishnan, Ishwar; He, Yuan; Imhoff, Rebecca; Sahu, Anirban
Citation:

Citation: He, Yuan; Imhoff, Rebecca; Sahu, Anirban; Radhakrishnan, Ishwar. "Solution Structure of a Novel Zinc Finger Motif in the SAP30 Polypeptide of the Sin3 Corepressor Complex and its Potential Role in Nucleic Acid Recognition"  Nucleic Acids Res. 37, 2145-2152 (2009).
PubMed: 19223330

Assembly members:

Assembly members:
SAP30_ZnF, polymer, 71 residues, 65.409 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMCSG7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SAP30_ZnF: SNAGQLCCLREDGERCGRAA GNASFSKRIQKSISQKKVKI ELDKSARHLYICDYHKNLIQ SVRNRRKRKGS

Data sets:
Data typeCount
13C chemical shifts297
15N chemical shifts78
1H chemical shifts443

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SAP30_ZnF1
2ZINC ION2

Entities:

Entity 1, SAP30_ZnF 71 residues - 65.409 Da.

1   SERASNALAGLYGLNLEUCYSCYSLEUARG
2   GLUASPGLYGLUARGCYSGLYARGALAALA
3   GLYASNALASERPHESERLYSARGILEGLN
4   LYSSERILESERGLNLYSLYSVALLYSILE
5   GLULEUASPLYSSERALAARGHISLEUTYR
6   ILECYSASPTYRHISLYSASNLEUILEGLN
7   SERVALARGASNARGARGLYSARGLYSGLY
8   SER

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: SAP30 ZnF, [U-95% 13C; U-95% 15N], 0.56 mM; TRIS, [U-13C; U-15N], 20 mM; acetic acid, [U-13C], 20 mM; DTT, [U-2H], 2 mM; Sodium Azide 0.2%; zinc chloride 0.56 mM; H2O 90%; D2O 10%

sample_2: SAP30 ZnF, [U-95% 13C; U-95% 15N], 0.56 mM; TRIS, [U-13C; U-15N], 20 mM; acetic acid, [U-13C], 20 mM; DTT, [U-2H], 2 mM; Sodium Azide 0.2%; zinc chloride 0.56 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.04 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

FELIX, Accelrys Software Inc. - data analysis, peak picking, processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, chemical shift calculation, data analysis

TALOS, Cornilescu, Delaglio and Bax - structure solution

ARIA, Linge, O'Donoghue and Nilges - refinement, structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

Procheck, Laskowski, MacArthur, Smith, Jones, Hutchinson, Morris, Moss and Tho - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB EDL87168 EDL87169 EGV91759 EHB04012 ELW67729
REF XP_003504367 XP_004389012 XP_004395295 XP_004433644 XP_005075210

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks