BMRB Entry 16121

Name: Structure of Archaeal L14e Ribosomal Protein from Sulfolobus sulfataricus
Published: 2009-05-20

Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16121

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of Archaeal L14e Ribosomal Protein from Sulfolobus sulfataricus

Deposition date:

2009-01-14

Original release date:

2009-05-20

Authors:

Edmondson, Stephen; Shriver, John

Citation:

Citation: Edmondson, Stephen; Turri, Jacquelyn; Smith, Kelley; Clark, Andrew; Shriver, John. "Structure, Stability, and Flexibility of Ribosomal Protein L14e from Sulfolobus solfataricus" Biochemistry 48, 5553-5562 (2009).
PubMed: 19432457

Assembly members:

Assembly members:
L14e_ribosomal_protein, polymer, 96 residues, 10881.970 Da.

Natural source:

Natural source: Common Name: Sulfolobus solfataricus Taxonomy ID: 2287 Superkingdom: Archaea Kingdom: not available Genus/species: Sulfolobus solfataricus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETBlue-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
L14e_ribosomal_protein: MPAIEVGRICVKVKGREAGS KCVIVDIIDDNFVLVTGPKD ITGVKRRRVNILHLEPTDKK IDIQKGASDEEVKKKLEESN LTEYMKEKIKIRMPTL

Data sets:

assigned_chemical_shifts
- assignments_sso8

Data type	Count
13C chemical shifts	420
15N chemical shifts	91
1H chemical shifts	684

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	L14e_ribosomal_protein	1

Entities:

Entity 1, L14e_ribosomal_protein 96 residues - 10881.970 Da.

1

MET

PRO

ALA

ILE

GLU

VAL

GLY

ARG

ILE

CYS

2

VAL

LYS

VAL

LYS

GLY

ARG

GLU

ALA

GLY

SER

3

LYS

CYS

VAL

ILE

VAL

ASP

ILE

ASP

4

ASN

PHE

VAL

LEU

VAL

THR

GLY

PRO

LYS

ASP

5

ILE

THR

GLY

VAL

LYS

ARG

VAL

ASN

6

ILE

LEU

HIS

LEU

GLU

PRO

THR

ASP

LYS

7

ILE

ASP

ILE

GLN

LYS

GLY

ALA

SER

ASP

GLU

8

GLU

VAL

LYS

LEU

GLU

SER

ASN

9

LEU

THR

GLU

TYR

MET

LYS

GLU

LYS

ILE

LYS

10

ILE

ARG

MET

PRO

THR

LEU

Samples:

sample_h2o: L14e ribosomal protein, [U-100% 13C; U-100% 15N], 1 mM; D2O 10%; H2O 90%

sample_d2o: L14e ribosomal protein, [U-100% 13C; U-100% 15N], 1 mM; D2O 100%

sample_n15: L14e ribosomal protein, [U-100% 15N], 1.0 mM; DSS 0.2 mM; D2O 10%; H2O 90%

sample_rdc: L14e ribosomal protein, [U-100% 15N], 1 mM; C12E5 5%; hexanol 1.0 r; D2O 10%; H2O 90%

sample_conditions_standard: ionic strength: 0 M; pH: 5.0; pressure: 1.0 atm; temperature: 303.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_n15	isotropic	sample_conditions_standard
3D HNCO	sample_h2o	isotropic	sample_conditions_standard
3D HNCACB	sample_h2o	isotropic	sample_conditions_standard
3D CBCA(CO)NH	sample_h2o	isotropic	sample_conditions_standard
3D HNCA	sample_h2o	isotropic	sample_conditions_standard
3D HN(CO)CA	sample_h2o	isotropic	sample_conditions_standard
3D HNHA	sample_h2o	isotropic	sample_conditions_standard
3D HCC_TOCSY-NNH	sample_h2o	isotropic	sample_conditions_standard
3D CCC-TOCSY-NNH	sample_h2o	isotropic	sample_conditions_standard
3D HCCH-TOCSY	sample_d2o	isotropic	sample_conditions_standard
3D HCCH-COSY	sample_d2o	isotropic	sample_conditions_standard
HBCBCGCDHD	sample_h2o	isotropic	sample_conditions_standard
2D DQF-COSY	sample_h2o	isotropic	sample_conditions_standard
2D 1H-1H NOESY	sample_h2o	isotropic	sample_conditions_standard
2D 1H-13C HSQC	sample_h2o	isotropic	sample_conditions_standard
3D 1H-15N NOESY	sample_h2o	isotropic	sample_conditions_standard
3D 1H-13C NOESY	sample_d2o	isotropic	sample_conditions_standard
2D 1H-15N HSQC	sample_d2o	isotropic	sample_conditions_standard
2D 1H-15N NOE	sample_h2o	isotropic	sample_conditions_standard
2D 1H-15N T1	sample_h2o	isotropic	sample_conditions_standard
2D 1H-15N T1rho	sample_h2o	isotropic	sample_conditions_standard
3D HNCO-IPAP	sample_rdc	anisotropic	sample_conditions_standard

Software:

VNMR, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView vaqua, Johnson, One Moon Scientific - chemical shift assignment, peak picking

ARIA v1.2, Linge, O'Donoghue and Nilges - chemical shift assignment

X-PLOR NIH v2.19, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

Varian INOVA 800 MHz
Varian INOVA 500 MHz

BMRB	15210
PDB	2JOY 2KDS
GB	AAK40722 ACX91605 AKA73699 AKA76396 AKA79089
REF	WP_009988801
SP	Q980C1
AlphaFold	Q980C1