BMRB Entry 16120

Name: Sequence-specific 1H, 13C, and 15N resonance assignment of the Autophagy-related Protein Atg 8
Published: 2009-04-14

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16120

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Sequence-specific 1H, 13C, and 15N resonance assignment of the Autophagy-related Protein Atg 8

Deposition date:

2009-01-14

Original release date:

2009-04-14

Authors:

Schwarten, Melanie; Stoldt, Matthias; Mohrluder, Jeannine; Willbold, Dieter

Citation:

Citation: Schwarten, Melanie; Stoldt, Matthias; Mohrluder, Jeannine; Willbold, Dieter. "Sequence-specific 1H, 13C, and 15N resonance assignment of the autophagy-related protein Atg8" Biomol. NMR Assignments 3, 137-139 (2009).
PubMed: 19636965

Assembly members:

Assembly members:
Atg8, polymer, 119 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGex-4T-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Atg8: GSMKSTFKSEYPFEKRKAES ERIADRFKNRIPVICEKAEK SDIPEIDKRKYLVPADLTVG QFVYVIRKRIMLPPEKAIFI FVNDTLPPTAALMSAIYQEH KDKDGFLYVTYSGENTFGR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	475
15N chemical shifts	106
1H chemical shifts	736

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Atg8	1

Entities:

Entity 1, Atg8 119 residues - Formula weight is not available

1

GLY

SER

MET

LYS

SER

THR

PHE

LYS

SER

GLU

2

TYR

PRO

PHE

GLU

LYS

ARG

LYS

ALA

GLU

SER

3

GLU

ARG

ILE

ALA

ASP

ARG

PHE

LYS

ASN

ARG

4

ILE

PRO

VAL

ILE

CYS

GLU

LYS

ALA

GLU

LYS

5

SER

ASP

ILE

PRO

GLU

ILE

ASP

LYS

ARG

LYS

6

TYR

LEU

VAL

PRO

ALA

ASP

LEU

THR

VAL

GLY

7

GLN

PHE

VAL

TYR

VAL

ILE

ARG

LYS

ARG

ILE

8

MET

LEU

PRO

GLU

LYS

ALA

ILE

PHE

ILE

9

PHE

VAL

ASN

ASP

THR

LEU

PRO

THR

ALA

10

ALA

LEU

MET

SER

ALA

ILE

TYR

GLN

GLU

HIS

11

LYS

ASP

LYS

ASP

GLY

PHE

LEU

TYR

VAL

THR

12

TYR

SER

GLY

GLU

ASN

THR

PHE

GLY

ARG

Samples:

sample_1: sodium phosphate 20 mM; sodium chloride 150 mM; DTT 5 mM; EDTA 1 mM; sodium azide 0.05%; Atg8, [U-13C; U-15N], 0.5 mM

sample_2: sodium phosphate 20 mM; sodium chloride 150 mM; DTT 5 mM; EDTA 1 mM; sodium azide 0.05%; Atg8, [U-13C; U-15N], 0.5 mM; DSS 1 mM

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
1D 1H	sample_2	isotropic	sample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA, Keller - chemical shift assignment

NMR spectrometers:

Varian VNMRS 800 MHz
Varian Unity_INOVA 600 MHz

BMRB	16835 17879
PDB	2KQ7 2KWC 2LI5 2ZPN 3RUI 3VH3 3VH4 3VXW
DBJ	GAA21477
EMBL	CAA56032 CAA84899 CAY77706
GB	AAT92889 AHY74413 AJP37015 AJP81917 AJP82297
REF	NP_009475
SP	A6ZKM4 P38182
TPG	DAA07045
AlphaFold	A6ZKM4 P38182