BMRB Entry 16115

Title:
Solution Structure of an SH3-like Domain from p80-Coilin
Deposition date:
2009-01-10
Original release date:
2011-06-01
Authors:
Donaldson, Logan
Citation:

Citation: Shanbhag, Riya; Kurabi, Arwa; Kwan, Jamie; Donaldson, Logan. "Solution structure of the carboxy-terminal Tudor domain from human Coilin."  FEBS Lett. 584, 4351-4356 (2010).
PubMed: 20875822

Assembly members:

Assembly members:
coilin, polymer, 141 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Data sets:
Data typeCount
13C chemical shifts323
15N chemical shifts71
1H chemical shifts465

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1coilin1

Entities:

Entity 1, coilin 141 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   GLYSERHISMETASPTYRSERLEULEUPRO
4   LEULEUALAALAALAPROGLNVALGLYGLU
5   LYSILEALAPHELYSLEULEUGLULEUTHR
6   SERSERTYRSERPROASPVALSERASPTYR
7   LYSGLUGLYARGILELEUSERHISASNPRO
8   GLUTHRGLNGLNVALASPILEGLUILELEU
9   SERSERLEUPROALALEUARGGLUPROGLY
10   LYSPHEASPLEUVALTYRHISASNGLUASN
11   GLYALAGLUVALVALGLUTYRALAVALTHR
12   GLNGLUSERLYSILETHRVALPHETRPLYS
13   GLULEUILEASPPROARGLEUILEILEGLU
14   SERPROSERASNTHRSERSERTHRGLUPRO
15   ALA

Samples:

sample: sodium phosphate 20 mM; sodium chloride 150 mM; sodium azide 0.05%; coilin; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 7.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D simultaneous 1H-13C-15N NOESYsampleisotropicsample_conditions_1
3D CBCA(CO)NHsampleisotropicsample_conditions_1
3D HNCOsampleisotropicsample_conditions_1
3D HNCACBsampleisotropicsample_conditions_1
3D H(CCO)NHsampleisotropicsample_conditions_1
3D C(CO)NHsampleisotropicsample_conditions_1
3D aromatic 1H-13C NOESYsampleisotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMRView, Johnson, One Moon Scientific - peak picking

TALOS, Cornilescu, Delaglio and Bax - dihedral restraints

NMR spectrometers:

  • Varian NMRS 600 MHz

Related Database Links:

DBJ BAE01330 BAG36378
GB AAA36412 AAB81550 AAH10385 ABM83853 ABM87175
REF NP_004636 XP_002827396 XP_009431163 XP_511900
SP P38432
AlphaFold P38432

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks