BMRB Entry 16115

Name: Solution Structure of an SH3-like Domain from p80-Coilin
Published: 2011-06-01

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16115

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of an SH3-like Domain from p80-Coilin

Deposition date:

2009-01-10

Original release date:

2011-06-01

Authors:

Donaldson, Logan

Citation:

Citation: Shanbhag, Riya; Kurabi, Arwa; Kwan, Jamie; Donaldson, Logan. "Solution structure of the carboxy-terminal Tudor domain from human Coilin." FEBS Lett. 584, 4351-4356 (2010).
PubMed: 20875822

Assembly members:

Assembly members:
coilin, polymer, 141 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
coilin: MGSSHHHHHHSSGLVPRGSH GSHMDYSLLPLLAAAPQVGE KIAFKLLELTSSYSPDVSDY KEGRILSHNPETQQVDIEIL SSLPALREPGKFDLVYHNEN GAEVVEYAVTQESKITVFWK ELIDPRLIIESPSNTSSTEP A

Data sets:

assigned_chemical_shifts
- assignments_coilin

Data type	Count
13C chemical shifts	323
15N chemical shifts	71
1H chemical shifts	465

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	coilin	1

Entities:

Entity 1, coilin 141 residues - Formula weight is not available

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

GLY

SER

HIS

MET

ASP

TYR

SER

LEU

PRO

4

LEU

ALA

PRO

GLN

VAL

GLY

GLU

5

LYS

ILE

ALA

PHE

LYS

LEU

GLU

LEU

THR

6

SER

TYR

SER

PRO

ASP

VAL

SER

ASP

TYR

7

LYS

GLU

GLY

ARG

ILE

LEU

SER

HIS

ASN

PRO

8

GLU

THR

GLN

VAL

ASP

ILE

GLU

ILE

LEU

9

SER

LEU

PRO

ALA

LEU

ARG

GLU

PRO

GLY

10

LYS

PHE

ASP

LEU

VAL

TYR

HIS

ASN

GLU

ASN

11

GLY

ALA

GLU

VAL

GLU

TYR

ALA

VAL

THR

12

GLN

GLU

SER

LYS

ILE

THR

VAL

PHE

TRP

LYS

13

GLU

LEU

ILE

ASP

PRO

ARG

LEU

ILE

GLU

14

SER

PRO

SER

ASN

THR

SER

THR

GLU

PRO

15

ALA

Samples:

sample: sodium phosphate 20 mM; sodium chloride 150 mM; sodium azide 0.05%; coilin; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 7.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D simultaneous 1H-13C-15N NOESY	sample	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample	isotropic	sample_conditions_1
3D HNCO	sample	isotropic	sample_conditions_1
3D HNCACB	sample	isotropic	sample_conditions_1
3D H(CCO)NH	sample	isotropic	sample_conditions_1
3D C(CO)NH	sample	isotropic	sample_conditions_1
3D aromatic 1H-13C NOESY	sample	isotropic	sample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMRView, Johnson, One Moon Scientific - peak picking

TALOS, Cornilescu, Delaglio and Bax - dihedral restraints

NMR spectrometers:

Varian NMRS 600 MHz

DBJ	BAE01330 BAG36378
GB	AAA36412 AAB81550 AAH10385 ABM83853 ABM87175
REF	NP_004636 XP_002827396 XP_009431163 XP_511900
SP	P38432
AlphaFold	P38432