BMRB Entry 16103

Assembly:

Entities:

Entity 1, FAIM-CTD monomer 94 residues - Formula weight is not available

Residues 1-5 are derived from a non-native protease site and polypeptide linker

Samples:

sample_1: FAIM-CTD, [U-99% 13C; U-99% 15N], 0.25 – 0.5 mM; sodium chloride 10 mM; TRIS 10 mM; DTT 5 mM

sample_2: FAIM-CTD, [U-99% 13C; U-99% 15N], 0.25 mM; sodium chloride 10 mM; TRIS 10 mM; DTT 5 mM; Pf1 filamentous phage 0.6% w/v

sample_3: FAIM-CTD, [U-99% 13C], 1.8 mM; sodium chloride 10 mM; TRIS 10 mM; DTT 5 mM

sample_4: FAIM-CTD, [U-10% 13C], 0.5 mM; sodium chloride 10 mM; TRIS 10 mM; DTT 5 mM

sample_5: FAIM-CTD, [U-99% 15N], 0.25 – 0.9 mM; sodium chloride 10 mM; TRIS 10 mM; DTT 5 mM

sample_6: FAIM-CTD 1.4 mM; sodium chloride 10 mM; TRIS 10 mM; DTT 5 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.3; pressure: 1.0 atm; temperature: 298 K

Experiments:

Software:

NMRPipe v2.4 Rev 2006.095.11.35, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.110, Goddard - data analysis

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

• Varian INOVA 500 MHz
• Bruker Avance 900 MHz