BMRB Entry 16089

Name: Solution NMR structure of ribosomal protein sso0164 from Sulfolobus solfataricus. NortheastStructural Genomics Consortium (NESG) target SsT4
Published: 2010-09-24

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PDB ID: 2kco
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16089
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of ribosomal protein sso0164 from Sulfolobus solfataricus. NortheastStructural Genomics Consortium (NESG) target SsT4

Deposition date:

2008-12-23

Original release date:

2010-09-24

Authors:

Lemak, Alexander; Gutmanas, Aleksandras; Yee, Adelinda; Fares, Christophe; Garcia, Maite; Montelione, Gaetano; Arrowsmith, Cheryl

Citation:

Citation: Lemak, Alexander; Gutmanas, Aleksandras; Yee, Adelinda; Fares, Christophe; Garcia, Maite; Montelione, Gaetano; Arrowsmith, Cheryl. "Solution NMR Structure of ribosomal protein sso0164 from Sulfolobus solfataricus" .

Assembly members:

Assembly members:
sso0164, polymer, 133 residues, 14686.104 Da.

Natural source:

Natural source: Common Name: Sulfolobus solfataricus Taxonomy ID: 2287 Superkingdom: Archaea Kingdom: not available Genus/species: Sulfolobus solfataricus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: P11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
sso0164: MGFYQGPDNRKITGGLKGKH RDKRKYEIGNPPTFTTLSAE DIRIKDRTLGGNFKVRLKYT TTANVLDPATNTAKKVKILE ILETPANKELARRGIIIRGA KIRTEAGLAVVTSRPGQDGV INAVLLKNESQRS

Data sets:

RDCs
- RDC_list_1
assigned_chemical_shifts
- assigned_chem_shift_list_1
spectral_peak_list
- spectral_peak_list_1
- spectral_peak_list_2

Data type	Count
13C chemical shifts	478
15N chemical shifts	112
1H chemical shifts	817
residual dipolar couplings	30

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	sso0164	1

Entities:

Entity 1, sso0164 133 residues - 14686.104 Da.

1

MET

GLY

PHE

TYR

GLN

GLY

PRO

ASP

ASN

ARG

2

LYS

ILE

THR

GLY

LEU

LYS

GLY

LYS

HIS

3

ARG

ASP

LYS

ARG

LYS

TYR

GLU

ILE

GLY

ASN

4

PRO

THR

PHE

THR

LEU

SER

ALA

GLU

5

ASP

ILE

ARG

ILE

LYS

ASP

ARG

THR

LEU

GLY

6

GLY

ASN

PHE

LYS

VAL

ARG

LEU

LYS

TYR

THR

7

THR

ALA

ASN

VAL

LEU

ASP

PRO

ALA

THR

8

ASN

THR

ALA

LYS

VAL

LYS

ILE

LEU

GLU

9

ILE

LEU

GLU

THR

PRO

ALA

ASN

LYS

GLU

LEU

10

ALA

ARG

GLY

ILE

ARG

GLY

ALA

11

LYS

ILE

ARG

THR

GLU

ALA

GLY

LEU

ALA

VAL

12

VAL

THR

SER

ARG

PRO

GLY

GLN

ASP

GLY

VAL

13

ILE

ASN

ALA

VAL

LEU

LYS

ASN

GLU

SER

14

GLN

ARG

SER

Samples:

sample_1: sso0164, [U-13C; U-15N], 0.5 mM; TRIS 10 mM; sodium chloride 500 mM; ZnSO4 10 uM; DTT 10 mM; NaN3 0.01%; benzamidine 10 mM

sample_conditions_1: ionic strength: 500 mM; pH: 7.7; pressure: 1.0 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C_arom NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC(IPAP)	sample_1	anisotropic	sample_conditions_1

Software:

SPARKY, Goddard - peak picking

FMC, Lemak, Steren, Llinas, Arrowsmith - resonanmce assignment

TALOS, Cornilescu, Delaglio and Bax - data analysis

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 800 MHz
Varian INOVA 600 MHz
Bruker Avance 600 MHz

PDB	2KCO
GB	AAK40509 ACP36129 ACP46351 ACP47944 ACP55921
REF	WP_009990388 WP_012714112
SP	C3MJ42 C3MZT9 C3N864 C3NF48 C4KJ22
AlphaFold	C3MJ42 C3MZT9 C3N864 C3NF48 C4KJ22