BMRB Entry 16085

Name: 1H, 13C, 15N Chemical Shift Assignment for Saccharomyces cerevisiae Acyl Carrier Protein.
Published: 2009-04-16

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PDB ID: 2ml8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16085
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, 15N Chemical Shift Assignment for Saccharomyces cerevisiae Acyl Carrier Protein.

Deposition date:

2008-12-23

Original release date:

2009-04-16

Authors:

Perez, Daniel

Citation:

Citation: Perez, Daniel; Wider, Gerhard. "1H, 15N, 13C resonance assignment of the acyl carrier protein subunit of the Saccharomyces cerevisiae fatty acid synthase" Biomol. NMR Assignments 3, 133-136 (2009).
PubMed: 19636964

Assembly members:

Assembly members:
Acyl_Carrier_Protein, polymer, 183 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Acyl_Carrier_Protein: MGSSHHHHHHENLYFQSNAE IADEPVKASLLLHVLVAHKL KKSLDSIPMSKTIKDLVGGK STVQNEILGDLGKEFGTTPE KPEETPLEELAETFQDTFSG ALGKQSSSLLSRLISSKMPG GFTITVARKYLQTRWGLPSG RQDGVLLVALSNEPAARLGS EADAKAFLDSMAQKYASIVG VDL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	725
15N chemical shifts	169
1H chemical shifts	1181

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ACP single polypeptide chain	1

Entities:

Entity 1, ACP single polypeptide chain 183 residues - Formula weight is not available

1

MET

GLY

SER

HIS

2

GLU

ASN

LEU

TYR

PHE

GLN

SER

ASN

ALA

GLU

3

ILE

ALA

ASP

GLU

PRO

VAL

LYS

ALA

SER

LEU

4

LEU

HIS

VAL

LEU

VAL

ALA

HIS

LYS

LEU

5

LYS

SER

LEU

ASP

SER

ILE

PRO

MET

SER

6

LYS

THR

ILE

LYS

ASP

LEU

VAL

GLY

LYS

7

SER

THR

VAL

GLN

ASN

GLU

ILE

LEU

GLY

ASP

8

LEU

GLY

LYS

GLU

PHE

GLY

THR

PRO

GLU

9

LYS

PRO

GLU

THR

PRO

LEU

GLU

LEU

10

ALA

GLU

THR

PHE

GLN

ASP

THR

PHE

SER

GLY

11

ALA

LEU

GLY

LYS

GLN

SER

LEU

12

SER

ARG

LEU

ILE

SER

LYS

MET

PRO

GLY

13

GLY

PHE

THR

ILE

THR

VAL

ALA

ARG

LYS

TYR

14

LEU

GLN

THR

ARG

TRP

GLY

LEU

PRO

SER

GLY

15

ARG

GLN

ASP

GLY

VAL

LEU

VAL

ALA

LEU

16

SER

ASN

GLU

PRO

ALA

ARG

LEU

GLY

SER

17

GLU

ALA

ASP

ALA

LYS

ALA

PHE

LEU

ASP

SER

18

MET

ALA

GLN

LYS

TYR

ALA

SER

ILE

VAL

GLY

19

VAL

ASP

LEU

Samples:

sample_1: Acyl Carrier Protein, [U-13C; U-15N], 1 mM; KH2PO4 : K2HPO4 (0.685 : 0.315) buffer 52 mM

sample_conditions_1: ionic strength: 0.052 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCA	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

CARA, Keller and Wuthrich - data analysis

NMR spectrometers:

Bruker DRX 750 MHz

PDB	2ML8 2UV8 2VKZ 3HMJ
DBJ	GAA26747
EMBL	CAA54218 CAA64256 CAA97948 CAY86729
GB	AAA34601 AHY77952 AJP41920 AJU23364 AJU24052
REF	NP_015093
SP	P19097
TPG	DAA11205
AlphaFold	P19097