BMRB Entry 16076

Name: Mouse prion protein fragment mPrP[P102L](91-231) at pH 7.0
Published: 2009-06-26

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16076

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Mouse prion protein fragment mPrP[P102L](91-231) at pH 7.0

Deposition date:

2008-12-22

Original release date:

2009-06-26

Authors:

Damberger, Fred; Wuthrich, Kurt; Hornemann, Simone; von Schroetter, Christine

Citation:

Citation: Hornemann, Simone; von Schroetter, Christine; Damberger, Fred; Wuthrich, Kurt. "Prion protein-detergent micelle interactions studied by NMR in solution." J. Biol. Chem. 284, 22713-22721 (2009).
PubMed: 19546219

Assembly members:

Assembly members:
mPrP90_P102L, polymer, 141 residues, 16163.8 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRSET A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
mPrP90_P102L: QGGGTHNQWNKLSKPKTNLK HVAGAAAAGAVVGGLGGYML GSAMSRPMIHFGNDWEDRYY RENMYRYPNQVYYRPVDQYS NQNNFVHDCVNITIKQHTVT TTTKGENFTETDVKMMERVV EQMCVTQYQKESQAYYDGRR S

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	268
15N chemical shifts	127
1H chemical shifts	127

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	mPrP90_P102L	1

Entities:

Entity 1, mPrP90_P102L 141 residues - 16163.8 Da.

1

GLN

GLY

THR

HIS

ASN

GLN

TRP

ASN

2

LYS

LEU

SER

LYS

PRO

LYS

THR

ASN

LEU

LYS

3

HIS

VAL

ALA

GLY

ALA

GLY

ALA

4

VAL

GLY

LEU

GLY

TYR

MET

LEU

5

GLY

SER

ALA

MET

SER

ARG

PRO

MET

ILE

HIS

6

PHE

GLY

ASN

ASP

TRP

GLU

ASP

ARG

TYR

7

ARG

GLU

ASN

MET

TYR

ARG

TYR

PRO

ASN

GLN

8

VAL

TYR

ARG

PRO

VAL

ASP

GLN

TYR

SER

9

ASN

GLN

ASN

PHE

VAL

HIS

ASP

CYS

VAL

10

ASN

ILE

THR

ILE

LYS

GLN

HIS

THR

VAL

THR

11

THR

LYS

GLY

GLU

ASN

PHE

THR

GLU

12

THR

ASP

VAL

LYS

MET

GLU

ARG

VAL

13

GLU

GLN

MET

CYS

VAL

THR

GLN

TYR

GLN

LYS

14

GLU

SER

GLN

ALA

TYR

ASP

GLY

ARG

15

SER

Samples:

sample_1: mPrP90_M129V, [U-99% 13C; U-99% 15N], 1.2 ± 0.05 mM; sodium phosphate buffer 5 mM

sample_conditions_1: ionic strength: 0.03 M; pH: 7.0; pressure: 1 atm; temperature: 293.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.8, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 500 MHz