BMRB Entry 16071

Name: Mouse prion protein fragment 90-231 at pH 7.0
Published: 2009-06-26

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16071

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Mouse prion protein fragment 90-231 at pH 7.0

Deposition date:

2008-12-19

Original release date:

2009-06-26

Authors:

Damberger, Fred; Wuthrich, Kurt; Hornemann, Simone; von Schroetter, Christine

Citation:

Citation: Hornemann, Simone; von Schroetter, Christine; Damberger, Fred; Wuthrich, Kurt. "Prion protein-detergent micelle interactions studied by NMR in solution." J. Biol. Chem. 284, 22713-22721 (2009).
PubMed: 19546219

Assembly members:

Assembly members:
mPrP90, polymer, 144 residues, 164114.1 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRSET A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
mPrP90: GSGQGGGTHNQWNKPSKPKT NLKHVAGAAAAGAVVGGLGG YMLGSAMSRPMIHFGNDWED RYYRENMYRYPNQVYYRPVD QYSNQNNFVHDCVNITIKQH TVTTTTKGENFTETDVKMME RVVEQMCVTQYQKESQAYYD GRRS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	389
15N chemical shifts	127
1H chemical shifts	127

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	mPrP90	1

Entities:

Entity 1, mPrP90 144 residues - 164114.1 Da.

1

GLY

SER

GLY

GLN

GLY

THR

HIS

ASN

2

GLN

TRP

ASN

LYS

PRO

SER

LYS

PRO

LYS

THR

3

ASN

LEU

LYS

HIS

VAL

ALA

GLY

ALA

4

ALA

GLY

ALA

VAL

GLY

LEU

GLY

5

TYR

MET

LEU

GLY

SER

ALA

MET

SER

ARG

PRO

6

MET

ILE

HIS

PHE

GLY

ASN

ASP

TRP

GLU

ASP

7

ARG

TYR

ARG

GLU

ASN

MET

TYR

ARG

TYR

8

PRO

ASN

GLN

VAL

TYR

ARG

PRO

VAL

ASP

9

GLN

TYR

SER

ASN

GLN

ASN

PHE

VAL

HIS

10

ASP

CYS

VAL

ASN

ILE

THR

ILE

LYS

GLN

HIS

11

THR

VAL

THR

LYS

GLY

GLU

ASN

12

PHE

THR

GLU

THR

ASP

VAL

LYS

MET

GLU

13

ARG

VAL

GLU

GLN

MET

CYS

VAL

THR

GLN

14

TYR

GLN

LYS

GLU

SER

GLN

ALA

TYR

ASP

15

GLY

ARG

SER

Samples:

sample_1: mPrP90, [U-99% 13C; U-99% 15N], 1.2 ± 0.05 mM; H2O 90%; D2O 10%; Sodium Phosphate buffer at pH 7.0 5 mM

sample_conditions_1: ionic strength: 0.03 M; pH: 7.0; pressure: 1 atm; temperature: 293.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.8, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 500 MHz