BMRB Entry 16069

Name: Resonance assignments for PilZ from Xanthomonas citri
Published: 2009-02-04

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16069

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Resonance assignments for PilZ from Xanthomonas citri

Deposition date:

2008-12-18

Original release date:

2009-02-04

Authors:

Guzzo, Cristiane; Salinas, Roberto; Farah, Chuck

Citation:

Citation: Guzzo, Cristiane; Salinas, Roberto; Andrade, Maxuel; Farah, Chuck. "PILZ protein structure and interactions with PILB and the FIMX EAL domain: implications for control of type IV pilus biogenesis." J. Mol. Biol. 393, 848-866 (2009).
PubMed: 19646999

Assembly members:

Assembly members:
PilZ, polymer, 117 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Xanthomonas axonopodis Taxonomy ID: 53413 Superkingdom: Bacteria Kingdom: not available Genus/species: Xanthomonas axonopodis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PilZ: MSAMNARQGILSLALKDKPA LYSAYMPFVKGGGIFVPTPK RYMLGDEVFLLLTLPDSSER LPVAGKVIWTTPAGAQGNRA AGIGVQFPDGPEGEAVRNKI ETLLAGLTTSDKPTHTM

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
heteronucl_NOEs
- 1H-15N_NOE

Data type	Count
13C chemical shifts	184
15N chemical shifts	93
1H chemical shifts	93
heteronuclear NOE values	87

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PilZ	1

Entities:

Entity 1, PilZ 117 residues - Formula weight is not available

1

MET

SER

ALA

MET

ASN

ALA

ARG

GLN

GLY

ILE

2

LEU

SER

LEU

ALA

LEU

LYS

ASP

LYS

PRO

ALA

3

LEU

TYR

SER

ALA

TYR

MET

PRO

PHE

VAL

LYS

4

GLY

ILE

PHE

VAL

PRO

THR

PRO

LYS

5

ARG

TYR

MET

LEU

GLY

ASP

GLU

VAL

PHE

LEU

6

LEU

THR

LEU

PRO

ASP

SER

GLU

ARG

7

LEU

PRO

VAL

ALA

GLY

LYS

VAL

ILE

TRP

THR

8

THR

PRO

ALA

GLY

ALA

GLN

GLY

ASN

ARG

ALA

9

ALA

GLY

ILE

GLY

VAL

GLN

PHE

PRO

ASP

GLY

10

PRO

GLU

GLY

GLU

ALA

VAL

ARG

ASN

LYS

ILE

11

GLU

THR

LEU

ALA

GLY

LEU

THR

SER

12

ASP

LYS

PRO

THR

HIS

THR

MET

Samples:

sample_1: PilZ, [U-99% 13C; U-99% 15N], 0.5 mM; Tris buffer 5 mM; NaCl 150 mM

sample_2: PilZ, [U-99% 15N], 0.5 ± 0.05 mM; Tris buffer 5 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 0.150 M; pH: 7.0; pressure: 1 atm; temperature: 300 K

sample_conditions_2: ionic strength: 0.150 M; pH: 7.0; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
1H-15N NOE	sample_2	isotropic	sample_conditions_2

Software:

Analysis_CCPN v1.0.14, CCPN - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz
Bruker DRX 500 MHz

PDB	3CNR 4F48 4FOU
DBJ	BAE67567 GAE49623 GAE57138 GAE61747
EMBL	CAJ22783 CAP52699 CCF68085 CCG36105 CDF60728
GB	AAM36006 AAM40324 AAW74141 AAY50265 ACD60951
REF	NP_636400 WP_002814313 WP_003483889 WP_007969015 WP_011036225