BMRB Entry 16059
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16059
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Piserchio, Andrea; Nair, Pravin; Shuman, Stewart; Ghose, Ranajeet. "Sequence-specific 1H N, 13C, and 15N backbone resonance assignments of the 34 kDa Paramecium bursaria Chlorella virus 1 (PBCV1) DNA ligase" Biomol. NMR Assignments 3, 77-80 (2009).
PubMed: 19636951
Assembly members:
Chv_DNA_Ligase, polymer, 319 residues, Formula weight is not available
AMP, non-polymer, 347.221 Da.
Natural source: Common Name: Chlorella virus Taxonomy ID: 10507 Superkingdom: Viruses Kingdom: not available Genus/species: Chlorovirus Chlorella virus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Pet
Entity Sequences (FASTA):
Chv_DNA_Ligase: MGHHHHHHHHHHSSGHIEGR
HMAITKPLLAATLENIEDVQ
FPCLATPKIDGIRSVKQTQM
LSRTFKPIRNSVMNRLLTEL
LPEGSDGEISIEGATFQDTT
SAVMTGHKMYNAKFSYYWFD
YVTDDPLKKYIDRVEDMKNY
ITVHPHILEHAQVKIIPLIP
VEINNITELLQYERDVLSKG
FEGVMIRKPDGKYKFGRSTL
KEGILLKMKQFKDAEATIIS
MTALFKNTNTKTKDNFGYSK
RSTHKSGKVEEDVMGSIEVD
YDGVVFSIGTGFDADQRRDF
WQNKESYIGKMVKFKYFEMG
SKDCPRFPVFIGIRHEEDR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 733 |
| 15N chemical shifts | 248 |
| 1H chemical shifts | 248 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Chv DNA Ligase | 1 |
| 2 | adenosine monophosphate | 2 |
Entities:
Entity 1, Chv DNA Ligase 319 residues - Formula weight is not available
Residues 1-21 are a cloning artifact. Numbering starts from position 22 (M1, A2, I3, T4 ..)
| 1 | MET | GLY | HIS | HIS | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | HIS | HIS | SER | SER | GLY | HIS | ILE | GLU | GLY | ARG | ||||
| 3 | HIS | MET | ALA | ILE | THR | LYS | PRO | LEU | LEU | ALA | ||||
| 4 | ALA | THR | LEU | GLU | ASN | ILE | GLU | ASP | VAL | GLN | ||||
| 5 | PHE | PRO | CYS | LEU | ALA | THR | PRO | LYS | ILE | ASP | ||||
| 6 | GLY | ILE | ARG | SER | VAL | LYS | GLN | THR | GLN | MET | ||||
| 7 | LEU | SER | ARG | THR | PHE | LYS | PRO | ILE | ARG | ASN | ||||
| 8 | SER | VAL | MET | ASN | ARG | LEU | LEU | THR | GLU | LEU | ||||
| 9 | LEU | PRO | GLU | GLY | SER | ASP | GLY | GLU | ILE | SER | ||||
| 10 | ILE | GLU | GLY | ALA | THR | PHE | GLN | ASP | THR | THR | ||||
| 11 | SER | ALA | VAL | MET | THR | GLY | HIS | LYS | MET | TYR | ||||
| 12 | ASN | ALA | LYS | PHE | SER | TYR | TYR | TRP | PHE | ASP | ||||
| 13 | TYR | VAL | THR | ASP | ASP | PRO | LEU | LYS | LYS | TYR | ||||
| 14 | ILE | ASP | ARG | VAL | GLU | ASP | MET | LYS | ASN | TYR | ||||
| 15 | ILE | THR | VAL | HIS | PRO | HIS | ILE | LEU | GLU | HIS | ||||
| 16 | ALA | GLN | VAL | LYS | ILE | ILE | PRO | LEU | ILE | PRO | ||||
| 17 | VAL | GLU | ILE | ASN | ASN | ILE | THR | GLU | LEU | LEU | ||||
| 18 | GLN | TYR | GLU | ARG | ASP | VAL | LEU | SER | LYS | GLY | ||||
| 19 | PHE | GLU | GLY | VAL | MET | ILE | ARG | LYS | PRO | ASP | ||||
| 20 | GLY | LYS | TYR | LYS | PHE | GLY | ARG | SER | THR | LEU | ||||
| 21 | LYS | GLU | GLY | ILE | LEU | LEU | LYS | MET | LYS | GLN | ||||
| 22 | PHE | LYS | ASP | ALA | GLU | ALA | THR | ILE | ILE | SER | ||||
| 23 | MET | THR | ALA | LEU | PHE | LYS | ASN | THR | ASN | THR | ||||
| 24 | LYS | THR | LYS | ASP | ASN | PHE | GLY | TYR | SER | LYS | ||||
| 25 | ARG | SER | THR | HIS | LYS | SER | GLY | LYS | VAL | GLU | ||||
| 26 | GLU | ASP | VAL | MET | GLY | SER | ILE | GLU | VAL | ASP | ||||
| 27 | TYR | ASP | GLY | VAL | VAL | PHE | SER | ILE | GLY | THR | ||||
| 28 | GLY | PHE | ASP | ALA | ASP | GLN | ARG | ARG | ASP | PHE | ||||
| 29 | TRP | GLN | ASN | LYS | GLU | SER | TYR | ILE | GLY | LYS | ||||
| 30 | MET | VAL | LYS | PHE | LYS | TYR | PHE | GLU | MET | GLY | ||||
| 31 | SER | LYS | ASP | CYS | PRO | ARG | PHE | PRO | VAL | PHE | ||||
| 32 | ILE | GLY | ILE | ARG | HIS | GLU | GLU | ASP | ARG |
Entity 2, adenosine monophosphate - C10 H14 N5 O7 P - 347.221 Da.
| 1 | AMP |
Samples:
sample_1: Chv DNA Ligase, [U-13C; U-15N; U-2H], 0.3 mM; adenosine monophosphate 0.3 mM; MES 20 mM; sodium chloride 300 mM; DTT 2 mM; EDTA 1 mM
sample_conditions_1: ionic strength: 0.302 M; pH: 6.5; pressure: 1 atm; temperature: 298.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks