BMRB Entry 16051

Name: Solution NMR structure of the OB domain (residues 67-166) of MM0293 from Methanosarcina mazei. Northeast Structural Genomics Consortium target MaR214a.
Published: 2011-02-24

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PDB ID: 2kbn
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16051
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of the OB domain (residues 67-166) of MM0293 from Methanosarcina mazei. Northeast Structural Genomics Consortium target MaR214a.

Deposition date:

2008-12-03

Original release date:

2011-02-24

Authors:

Ramelot, Theresa; Ding, Keyang; Magliqui, Melissa; Jiang, Mei; Ciccosanti, Colleen; Xiao, Rong; Lui, Jinfeng; Everett, John; Swapna, G.V.T.; Acton, Thomas; Rost, Burkhardt; Montelione, Gaetano; Kennedy, Michael

Citation:

Citation: Kennedy, Michael; Ramelot, Theresa; Ding, Keyang; Magliqui, Melissa; Jiang, Mei; Ciccosanti, Colleen; Xiao, Rong; Lui, Jinfeng; Everett, John; Swapna, G.V.T.; Acton, Thomas; Rost, Burkhardt; Montelione, Gaetano. "NMR structure of the OB domain of mm0293 from the archea methanosarcina mazei" .

Assembly members:

Assembly members:
MM0293, polymer, 109 residues, 12519.227 Da.

Natural source:

Natural source: Common Name: Methanosarcina mazei Taxonomy ID: 2209 Superkingdom: Archaea Kingdom: not available Genus/species: Methanosarcina mazei

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21-23C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MM0293: MEPQLTKIVDIVENGQWANL KAKVIQLWENTHESISQVGL LGDETGIIKFTIWKNAELPL LEQGESYLLRSVVVGEYNDR FQVQVNKNSSIEKLSEPIEV GLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	463
15N chemical shifts	119
1H chemical shifts	769

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MM0293	1

Entities:

Entity 1, MM0293 109 residues - 12519.227 Da.

Residues 2-100 correspond to residues 67-166 in the full-length protein. Residues 101-109 correspond to residues associated with the C-terminal His tag.

1

MET

GLU

PRO

GLN

LEU

THR

LYS

ILE

VAL

ASP

2

ILE

VAL

GLU

ASN

GLY

GLN

TRP

ALA

ASN

LEU

3

LYS

ALA

LYS

VAL

ILE

GLN

LEU

TRP

GLU

ASN

4

THR

HIS

GLU

SER

ILE

SER

GLN

VAL

GLY

LEU

5

LEU

GLY

ASP

GLU

THR

GLY

ILE

LYS

PHE

6

THR

ILE

TRP

LYS

ASN

ALA

GLU

LEU

PRO

LEU

7

LEU

GLU

GLN

GLY

GLU

SER

TYR

LEU

ARG

8

SER

VAL

GLY

GLU

TYR

ASN

ASP

ARG

9

PHE

GLN

VAL

GLN

VAL

ASN

LYS

ASN

SER

10

ILE

GLU

LYS

LEU

SER

GLU

PRO

ILE

GLU

VAL

11

GLY

LEU

GLU

HIS

Samples:

sample_1: OB domain, [U-100% 13C; U-100% 15N], 1.2 mM; MES 20 mM; sodium chloride 100 mM; DTT 10 mM; calcium chloride 5 mM; sodium azide 0.02%

sample_2: OB domain, [U-5% 13C; U-99% 15N], 1.2 mM; MES 20 mM; sodium chloride 100 mM; DTT 10 mM; calcium chloride 5 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
4D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection

SPARKY v3.113, Goddard - data analysis

AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

AutoStruct v2.2.1, Huang, Tejero, Powers and Montelione - structure solution

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - data analysis

PSVS v1.3, Bhattacharya and Montelione - data analysis

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

X-PLOR NIH v2.2.1, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

Bruker Avance 850 MHz
Varian INOVA 600 MHz