BMRB Entry 16044

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16044

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Title:
N-terminal backbone assignment of the polyoma virus large T antigen
Deposition date:
2008-11-27
Original release date:
2009-04-16
Authors:
Knoblich, Konstantin; Jiang, Tao; Schaffhausen, Brian; Gunther, Ulrich
Citation:

Citation: Knoblich, Konstantin; Whittaker, Sara; Ludwig, Christian; Michiels, Paul; Jiang, Tao; Schaffhausen, Brian; Gunther, Ulrich. "Backbone assignment of the N-terminal polyomavirus large T antigen"  Biomol. NMR Assignments 3, 119-123 (2009).
PubMed: 19636961

Assembly members:

Assembly members:
PyLTNT, polymer, 264 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Murine polyomavirus   Taxonomy ID: 10634   Superkingdom: Viruses   Kingdom: not available   Genus/species: Polyomavirus Murine polyomavirus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-30b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PyLTNT: MDRVLSRADKERLLELLKLP RQLWGDFGRMQQAYKQQSLL LHPDKGGSHALMQELNSLWG TFKTEVYNLRMNLGGTGFQG SPPRTAERGTEESGHSPLHD DYWSFSYGSKYFTREWNDFF RKWDPSYQSPPKTAESSEQP DLFCYEEPLLSPNPSSPTDT PAHTAGRRRNPCVAEPDDSI SPDPPRTPVSRKRPRPAGAT GGGGGGVHANGGSVFGHPTG GTSTPAHPPPYHSQGGSESM GGSDSSGFAEGSFRSDGSHH HHHH

Data sets:
Data typeCount
13C chemical shifts406
15N chemical shifts142
1H chemical shifts261

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PyLTNT1

Entities:

Entity 1, PyLTNT 264 residues - Formula weight is not available

1   METASPARGVALLEUSERARGALAASPLYS
2   GLUARGLEULEUGLULEULEULYSLEUPRO
3   ARGGLNLEUTRPGLYASPPHEGLYARGMET
4   GLNGLNALATYRLYSGLNGLNSERLEULEU
5   LEUHISPROASPLYSGLYGLYSERHISALA
6   LEUMETGLNGLULEUASNSERLEUTRPGLY
7   THRPHELYSTHRGLUVALTYRASNLEUARG
8   METASNLEUGLYGLYTHRGLYPHEGLNGLY
9   SERPROPROARGTHRALAGLUARGGLYTHR
10   GLUGLUSERGLYHISSERPROLEUHISASP
11   ASPTYRTRPSERPHESERTYRGLYSERLYS
12   TYRPHETHRARGGLUTRPASNASPPHEPHE
13   ARGLYSTRPASPPROSERTYRGLNSERPRO
14   PROLYSTHRALAGLUSERSERGLUGLNPRO
15   ASPLEUPHECYSTYRGLUGLUPROLEULEU
16   SERPROASNPROSERSERPROTHRASPTHR
17   PROALAHISTHRALAGLYARGARGARGASN
18   PROCYSVALALAGLUPROASPASPSERILE
19   SERPROASPPROPROARGTHRPROVALSER
20   ARGLYSARGPROARGPROALAGLYALATHR
21   GLYGLYGLYGLYGLYGLYVALHISALAASN
22   GLYGLYSERVALPHEGLYHISPROTHRGLY
23   GLYTHRSERTHRPROALAHISPROPROPRO
24   TYRHISSERGLNGLYGLYSERGLUSERMET
25   GLYGLYSERASPSERSERGLYPHEALAGLU
26   GLYSERPHEARGSERASPGLYSERHISHIS
27   HISHISHISHIS

Samples:

sample_1: PyLTNT, [U-99% 15N], 700 uM; KCl 150 mM

sample_2: PyLTNT, [U-99% 13C; U-99% 15N], 700 uM; KCl 150 mM

sample_3: PyLTNT, [U-99% 13C; U-99% 15N], 400 uM; KCl 150 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HNNsample_2isotropicsample_conditions_1
3D HNCNsample_2isotropicsample_conditions_1
3D CBCACONsample_3isotropicsample_conditions_1
3D CBCANCOsample_3isotropicsample_conditions_1
2D CONsample_3isotropicsample_conditions_1

Software:

Analysis, CCPN - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 900 MHz
  • Varian INOVA 800 MHz

Related Database Links:

EMBL CAA42510
GB AAA46872 AAA93241 AAA93245 AAB59901 AAL35609
REF YP_009111413
SP P03073 P03074
AlphaFold P03073 P03074

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks