BMRB Entry 16043

Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16043

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Title:
The solution structure of Bacillus subtilis SR211 by NMR spectroscopy
Deposition date:
2008-11-25
Original release date:
2009-07-22
Authors:
Mercier, Kelly; Mueller, Geoffrey; Powers, Robert
Citation:

Citation: Mercier, Kelly; Mueller, Geoffrey; Acton, Thomas; Xiao, Rong; Montelione, Gaetano; Powers, Robert. "(1)H, (13)C, and (15)N NMR assignments for the Bacillus subtilis yndB START domain."  Biomol. NMR Assignments 3, 191-194 (2009).
PubMed: 19888688

Assembly members:

Assembly members:
SR211, polymer, 152 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: MVK

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SR211: MAQNNENALPDITKSITLEA PIQKVWETVSTSEGIAKWFM PNDFQLKEGQEFHLQSPFGP SPCKVLAVQAPTELSFEWDT EGWVVTFQLEDLGEKTGFTL IHSGWKEPNQVIGKANEKSS VVRGKMDGGWTGIVNERLRK AVEELEHHHHHH

Data sets:
Data typeCount
13C chemical shifts488
15N chemical shifts118
1H chemical shifts690

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SR2111

Entities:

Entity 1, SR211 152 residues - Formula weight is not available

1   METALAGLNASNASNGLUASNALALEUPRO
2   ASPILETHRLYSSERILETHRLEUGLUALA
3   PROILEGLNLYSVALTRPGLUTHRVALSER
4   THRSERGLUGLYILEALALYSTRPPHEMET
5   PROASNASPPHEGLNLEULYSGLUGLYGLN
6   GLUPHEHISLEUGLNSERPROPHEGLYPRO
7   SERPROCYSLYSVALLEUALAVALGLNALA
8   PROTHRGLULEUSERPHEGLUTRPASPTHR
9   GLUGLYTRPVALVALTHRPHEGLNLEUGLU
10   ASPLEUGLYGLULYSTHRGLYPHETHRLEU
11   ILEHISSERGLYTRPLYSGLUPROASNGLN
12   VALILEGLYLYSALAASNGLULYSSERSER
13   VALVALARGGLYLYSMETASPGLYGLYTRP
14   THRGLYILEVALASNGLUARGLEUARGLYS
15   ALAVALGLUGLULEUGLUHISHISHISHIS
16   HISHIS

Samples:

sample_1: SR211, [U-100% 13C; U-100% 15N], 1 mM; MES 20 mM; D2O 5%; H2O 95%; sodium azide 0.02%; DTT 10 mM; sodium chloride 100 mM; calcium chloride 5 mM

sample_conditions_1: ionic strength: .105 M; pH: 6.5; pressure: 1 atm; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - data analysis, refinement, structure solution

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Varian INOVA 750 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAI85452 BAM52425 BAM58001 GAK81575
EMBL CAB13657 CCU58384 CEI56962 CEJ77386 CJT03957
GB ADV92673 AFQ57707 AGA21279 AGE63615 AGG61147
REF NP_389656 WP_003231627 WP_015383753 WP_015713997 WP_017697365
SP O31806
AlphaFold O31806

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks