BMRB Entry 16042

Name: human brain-type fatty acid binding protein (hb-FABP
Published: 2009-03-11

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16042

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

human brain-type fatty acid binding protein (hb-FABP

Deposition date:

2008-11-25

Original release date:

2009-03-11

Authors:

Oeemig, Jesper; Jorgensen, Mathilde; Hansen, Mikka; Petersen, Evamaria; Duroux, Laurent; Wimmer, Reinhard

Citation:

Citation: Oeemig, Jesper; Jorgensen, Mathilde; Hansen, Mikka; Petersen, Evamaria; Duroux, Laurent; Wimmer, Reinhard. "Backbone and sidechain 1H, 13C and 15N resonance assignments of the human brain-type fatty acid binding protein (FABP7) in its apo form and the holo forms binding to DHA, oleic acid, linoleic acid and elaidic acid" Biomol. NMR Assignments 3, 89-93 (2009).
PubMed: 19636954

Assembly members:

Assembly members:
hb-FABP, polymer, 131 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hb-FABP: VEAFCATWKLTNSQNFDEYM KALGVGFATRQVGNVTKPTV IISQEGDKVVIRTLSTFKNT EISFQLGEEFDETTADDRNC KSVVSLDGDKLVHIQKWDGK ETNFVREIKDGKMVMTLTFG DVVAVRHYEKA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	369
15N chemical shifts	143
1H chemical shifts	923

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hb-FABP	1

Entities:

Entity 1, hb-FABP 131 residues - Formula weight is not available

1

VAL

GLU

ALA

PHE

CYS

ALA

THR

TRP

LYS

LEU

2

THR

ASN

SER

GLN

ASN

PHE

ASP

GLU

TYR

MET

3

LYS

ALA

LEU

GLY

VAL

GLY

PHE

ALA

THR

ARG

4

GLN

VAL

GLY

ASN

VAL

THR

LYS

PRO

THR

VAL

5

ILE

SER

GLN

GLU

GLY

ASP

LYS

VAL

6

ILE

ARG

THR

LEU

SER

THR

PHE

LYS

ASN

THR

7

GLU

ILE

SER

PHE

GLN

LEU

GLY

GLU

PHE

8

ASP

GLU

THR

ALA

ASP

ARG

ASN

CYS

9

LYS

SER

VAL

SER

LEU

ASP

GLY

ASP

LYS

10

LEU

VAL

HIS

ILE

GLN

LYS

TRP

ASP

GLY

LYS

11

GLU

THR

ASN

PHE

VAL

ARG

GLU

ILE

LYS

ASP

12

GLY

LYS

MET

VAL

MET

THR

LEU

THR

PHE

GLY

13

ASP

VAL

ALA

VAL

ARG

HIS

TYR

GLU

LYS

14

ALA

Samples:

sample_1: hb-FABP, [U-98% 13C; U-98% 15N], 0.85 mM; D2O 5%; H2O 95%; DSS 1 mM; TRIS 10 mM

sample_conditions_1: ionic strength: 0.01 M; pH: 7.4; pressure: 1 atm; temperature: 298.1 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

XEASY v1.3.13, Bartels et al. - data analysis

NMR spectrometers:

Bruker DRX 600 MHz

BMRB	5320 16046 16047 16048 16049
PDB	1JJX 1FDQ 1FE3 1JJX
DBJ	BAA23324 BAA23645 BAD92052 BAE90445 BAF82525
EMBL	CAA05773 CAC21646 CAG33338
GB	AAB87141 AAD00507 AAH12299 AAY17257 ABM82363
REF	NP_001020400 NP_001244643 NP_001270567 NP_001437 XP_002746967
SP	O15540
AlphaFold	O15540