BMRB Entry 16031

Name: Nav1.5 C-terminal EF-Hand Domain
Published: 2009-01-20

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16031

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Nav1.5 C-terminal EF-Hand Domain

Deposition date:

2008-11-15

Original release date:

2009-01-20

Authors:

Miloushev, Vesselin; Levine, Joshua; Arbing, Mark; Hunt, John; Pitt, Geoff; Palmer, Arthur

Citation:

Citation: Miloushev, Vesselin; Levine, Joshua; Arbing, Mark; Hunt, John; Pitt, Geoff; Palmer, Arthur. "Solution structure of the NaV1.2 C-terminal EF-hand domain." J. Biol. Chem. 284, 6446-6454 (2009).
PubMed: 19129176

Assembly members:

Assembly members:
Nav1.5, polymer, 129 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Nav1.5: MGSSHHHHHHSSGLVPRGSH MASENFSVATEESTEPLSED DFDMFYEIWEKFDPEATQFI EYSVLSDFADALSEPLRIAK PNQISLINMDLPMVSGDRIH CMDILFAFTKRVLGESGEMD ALKIQMEEK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	296
15N chemical shifts	97
1H chemical shifts	327

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Nav1.5	1

Entities:

Entity 1, Nav1.5 129 residues - Formula weight is not available

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

ALA

SER

GLU

ASN

PHE

SER

VAL

ALA

THR

4

GLU

SER

THR

GLU

PRO

LEU

SER

GLU

ASP

5

ASP

PHE

ASP

MET

PHE

TYR

GLU

ILE

TRP

GLU

6

LYS

PHE

ASP

PRO

GLU

ALA

THR

GLN

PHE

ILE

7

GLU

TYR

SER

VAL

LEU

SER

ASP

PHE

ALA

ASP

8

ALA

LEU

SER

GLU

PRO

LEU

ARG

ILE

ALA

LYS

9

PRO

ASN

GLN

ILE

SER

LEU

ILE

ASN

MET

ASP

10

LEU

PRO

MET

VAL

SER

GLY

ASP

ARG

ILE

HIS

11

CYS

MET

ASP

ILE

LEU

PHE

ALA

PHE

THR

LYS

12

ARG

VAL

LEU

GLY

GLU

SER

GLY

GLU

MET

ASP

13

ALA

LEU

LYS

ILE

GLN

MET

GLU

LYS

Samples:

sample_1: Nav1.5, [U-98% 13C; U-98% 15N], 0.5 ± 0.05 mM; TRIS, [D-11 98 %], 20 ± 0.2 mM; glycine, [D-5 98 %], 100 ± 1 mM; EDTA, [D-16 98 %], 0.1 ± 0.01 mM; D2O 10 ± 0.5 %; H2O 90 ± 0.5 %; NaN3 0.02 ± 0.002 %

sample_conditions_1: ionic strength: 0.1 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe v2.4, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.110, Goddard - chemical shift assignment

NMR spectrometers:

Bruker DRX 500 MHz
Bruker DRX 600 MHz
Bruker Avance 700 MHz

BMRB	16045
PDB	2KBI 4DCK 4JQ0
DBJ	BAD12084 BAD12085 BAD92103 BAE27800 BAE27966
EMBL	CAB70096 CAC80974 CAD88248
GB	AAA42114 AAA58644 AAI40814 AAI44622 AAI72643
REF	NP_000326 NP_001002994 NP_001092874 NP_001092875 NP_001153632
SP	P15389 Q14524 Q9JJV9
TPG	DAA17127 DAA34921
AlphaFold	P15389 Q14524 Q9JJV9