BMRB Entry 16031

Title:
Nav1.5 C-terminal EF-Hand Domain
Deposition date:
2008-11-15
Original release date:
2009-01-20
Authors:
Miloushev, Vesselin; Levine, Joshua; Arbing, Mark; Hunt, John; Pitt, Geoff; Palmer, Arthur
Citation:

Citation: Miloushev, Vesselin; Levine, Joshua; Arbing, Mark; Hunt, John; Pitt, Geoff; Palmer, Arthur. "Solution structure of the NaV1.2 C-terminal EF-hand domain."  J. Biol. Chem. 284, 6446-6454 (2009).
PubMed: 19129176

Assembly members:

Assembly members:
Nav1.5, polymer, 129 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Data sets:
Data typeCount
13C chemical shifts296
15N chemical shifts97
1H chemical shifts327

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Nav1.51

Entities:

Entity 1, Nav1.5 129 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METALASERGLUASNPHESERVALALATHR
4   GLUGLUSERTHRGLUPROLEUSERGLUASP
5   ASPPHEASPMETPHETYRGLUILETRPGLU
6   LYSPHEASPPROGLUALATHRGLNPHEILE
7   GLUTYRSERVALLEUSERASPPHEALAASP
8   ALALEUSERGLUPROLEUARGILEALALYS
9   PROASNGLNILESERLEUILEASNMETASP
10   LEUPROMETVALSERGLYASPARGILEHIS
11   CYSMETASPILELEUPHEALAPHETHRLYS
12   ARGVALLEUGLYGLUSERGLYGLUMETASP
13   ALALEULYSILEGLNMETGLUGLULYS

Samples:

sample_1: Nav1.5, [U-98% 13C; U-98% 15N], 0.5 ± 0.05 mM; TRIS, [D-11 98 %], 20 ± 0.2 mM; glycine, [D-5 98 %], 100 ± 1 mM; EDTA, [D-16 98 %], 0.1 ± 0.01 mM; D2O 10 ± 0.5 %; H2O 90 ± 0.5 %; NaN3 0.02 ± 0.002 %

sample_conditions_1: ionic strength: 0.1 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe v2.4, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.110, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz
  • Bruker Avance 700 MHz

Related Database Links:

BMRB 16045
PDB
DBJ BAD12084 BAD12085 BAD92103 BAE27800 BAE27966
EMBL CAB70096 CAC80974 CAD88248
GB AAA42114 AAA58644 AAI40814 AAI44622 AAI72643
REF NP_000326 NP_001002994 NP_001092874 NP_001092875 NP_001153632
SP P15389 Q14524 Q9JJV9
TPG DAA17127 DAA34921
AlphaFold P15389 Q14524 Q9JJV9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks