BMRB Entry 16009

Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16009

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Title:
NMR resonance assignment of DnaE intein from Nostoc punctiforme
Deposition date:
2008-10-29
Original release date:
2009-01-15
Authors:
Heinamaki, Kimmo; Djupsjobacka, Janica; Oemig, Jesper; Iwai, Hideo
Citation:

Citation: Heinamaki, Kimmo; Oeemig, Jesper; Djupsjobacka, Janica; Iwai, Hideo. "NMR resonance assignment of DnaE intein from Nostoc punctiforme"  Biomol. NMR Assignments 3, 41-43 (2009).
PubMed: 19636943

Assembly members:

Assembly members:
intein_polypeptide, polymer, 139 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: cyanobacterium   Taxonomy ID: 272131   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Nostoc Nostoc punctiforme

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHYRSF53

Entity Sequences (FASTA):

Entity Sequences (FASTA):
intein_polypeptide: GGALSYETEILTVEYGLLPI GKIVEKRIECTVYSVDNNGN IYTQPVAQWHDRGEQEVFEY CLEDGSLIRATKDHKFMTVD GQMLPIDEIFERELDLMRVD NLPNIKIATRKYLGKQNVYD IGVERDHNFALKNGFIASN

Data sets:
Data typeCount
13C chemical shifts611
15N chemical shifts146
1H chemical shifts969

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1single chain intein1

Entities:

Entity 1, single chain intein 139 residues - Formula weight is not available

1   GLYGLYALALEUSERTYRGLUTHRGLUILE
2   LEUTHRVALGLUTYRGLYLEULEUPROILE
3   GLYLYSILEVALGLULYSARGILEGLUCYS
4   THRVALTYRSERVALASPASNASNGLYASN
5   ILETYRTHRGLNPROVALALAGLNTRPHIS
6   ASPARGGLYGLUGLNGLUVALPHEGLUTYR
7   CYSLEUGLUASPGLYSERLEUILEARGALA
8   THRLYSASPHISLYSPHEMETTHRVALASP
9   GLYGLNMETLEUPROILEASPGLUILEPHE
10   GLUARGGLULEUASPLEUMETARGVALASP
11   ASNLEUPROASNILELYSILEALATHRARG
12   LYSTYRLEUGLYLYSGLNASNVALTYRASP
13   ILEGLYVALGLUARGASPHISASNPHEALA
14   LEULYSASNGLYPHEILEALASERASN

Samples:

sample_1: intein_polypeptide, [U-100% 13C; U-100% 15N], 2 mM; sodium phosphate 10 mM

sample_2: intein_polypeptide, [U-20% 13C; U-100% 15N], 2 mM; sodium phosphate 10 mM

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNHBsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
CCCONHsample_1isotropicsample_conditions_1
HCCsample_1isotropicsample_conditions_1
CBCGCDHDsample_1isotropicsample_conditions_1
CBCGCDHDHEsample_1isotropicsample_conditions_1

Software:

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - peak picking

Analysis v1.0.19, CCPN - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB ACC83218 ADG44968 ADG44969 AIS22787 AIS22800
REF WP_012411174

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks