BMRB Entry 16003

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16003

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for brain abundant protein 1 (BASP1)
Deposition date:
2008-10-24
Original release date:
2008-12-03
Authors:
Schedlbauer, Andreas; Kontaxis, Georg; Konrat, Robert; Bister, Klaus
Citation:

Citation: Schedlbauer, Andreas. "1H 13C and 15N Assigned Chemical Backbone Shifts for BASP1"  Proc. Natl. Acad. Sci. U. S. A. ., .-..

Assembly members:

Assembly members:
BASP1, polymer, 244 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pBR322

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BASP1: MGGKLSKKKKGYSVNDEKAK DKDKKAEGAATEEEETPKEA EDAQQTTETTEVKENNKEEK VEKDAQVSANKTEEKEGEKE KTVTQEEAQKAEPEKSEAVV DAKVEPQKNNEQAPKQEEPA AASAPAASSEAPKTSEPSSD AKASQPSEATAPSKADDKSK EEGEAKKTEAPATPAAQETK SEVAPASDSKPSSSEAAPSS KETVAATAAPSSTAKASDPS APPEEAKPSEAPATNSDQTI AVQD

Data sets:
Data typeCount
13C chemical shifts470
15N chemical shifts158
1H chemical shifts158

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BASP11

Entities:

Entity 1, BASP1 244 residues - Formula weight is not available

1   METGLYGLYLYSLEUSERLYSLYSLYSLYS
2   GLYTYRSERVALASNASPGLULYSALALYS
3   ASPLYSASPLYSLYSALAGLUGLYALAALA
4   THRGLUGLUGLUGLUTHRPROLYSGLUALA
5   GLUASPALAGLNGLNTHRTHRGLUTHRTHR
6   GLUVALLYSGLUASNASNLYSGLUGLULYS
7   VALGLULYSASPALAGLNVALSERALAASN
8   LYSTHRGLUGLULYSGLUGLYGLULYSGLU
9   LYSTHRVALTHRGLNGLUGLUALAGLNLYS
10   ALAGLUPROGLULYSSERGLUALAVALVAL
11   ASPALALYSVALGLUPROGLNLYSASNASN
12   GLUGLNALAPROLYSGLNGLUGLUPROALA
13   ALAALASERALAPROALAALASERSERGLU
14   ALAPROLYSTHRSERGLUPROSERSERASP
15   ALALYSALASERGLNPROSERGLUALATHR
16   ALAPROSERLYSALAASPASPLYSSERLYS
17   GLUGLUGLYGLUALALYSLYSTHRGLUALA
18   PROALATHRPROALAALAGLNGLUTHRLYS
19   SERGLUVALALAPROALASERASPSERLYS
20   PROSERSERSERGLUALAALAPROSERSER
21   LYSGLUTHRVALALAALATHRALAALAPRO
22   SERSERTHRALALYSALASERASPPROSER
23   ALAPROPROGLUGLUALALYSPROSERGLU
24   ALAPROALATHRASNSERASPGLNTHRILE
25   ALAVALGLNASP

Samples:

sample_1: BASP1, [U-13C; U-15N], 2.0 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNNsample_1isotropicsample_conditions_1
3D HNCNsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 500 MHz

Related Database Links:

GB AAM77680
REF NP_989447 XP_003204865
SP P23614
AlphaFold P23614

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks