BMRB Entry 15997

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15997

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Title:
NMR STRUCTURE OF DIMERIZATION DOMAIN OF HUMAN RIBOSOMAL PROTEIN P2
Deposition date:
2008-10-22
Original release date:
2010-05-06
Authors:
Lee, K.; Chan, S.; Sze, K.; Zhu, G.; Shaw, P.; Wong, K.
Citation:

Citation: Lee, Ka-Ming; Yu, Conny Wing-Heng; Chan, Denise So-Bik; Chiu, Teddy Yu-Hin; Zhu, Guang; Sze, Kong-Hung; Shaw, Pang-Chui; Wong, Kam-Bo. "Solution structure of the dimerization domain of ribosomal protein P2 provides insights for the structural organization of eukaryotic stalk."  Nucleic Acids Res. 38, 5206-5216 (2010).
PubMed: 20385603

Assembly members:

Assembly members:
HUMAN_RIBOSOME_PROTEIN_P2, polymer, 70 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: HUMAN   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: HOMO SAPIENS

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: ESCHERICHIA COLI   Vector: PET3D

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HUMAN_RIBOSOME_PROTEIN_P2: AMRYVASYLLAALGGNSSPS AKDIKKILDSVGIEADDDRL NKVISELNGKNIEDVIAQGI GKLASVPAGG

Data sets:
Data typeCount
13C chemical shifts210
15N chemical shifts67
1H chemical shifts577

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HUMAN RIBOSOME PROTEIN P2_11
2HUMAN RIBOSOME PROTEIN P2_21

Entities:

Entity 1, HUMAN RIBOSOME PROTEIN P2_1 70 residues - Formula weight is not available

1   ALAMETARGTYRVALALASERTYRLEULEU
2   ALAALALEUGLYGLYASNSERSERPROSER
3   ALALYSASPILELYSLYSILELEUASPSER
4   VALGLYILEGLUALAASPASPASPARGLEU
5   ASNLYSVALILESERGLULEUASNGLYLYS
6   ASNILEGLUASPVALILEALAGLNGLYILE
7   GLYLYSLEUALASERVALPROALAGLYGLY

Samples:

sample_1: HUMAN RIBOSOME PROTEIN P2, [U-13C; U-15N], 1 mM; H2O 90%; D2O 10%; SODIUM SULFATE 200 mM

sample_conditions_1: ionic strength: 200 mM; pH: 7.5; pressure: 1.0 atm; temperature: 298.0 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1isotropicsample_conditions_1
COSYsample_1isotropicsample_conditions_1
TOCSYsample_1isotropicsample_conditions_1

Software:

CNS, BRUNGER,ADAMS,CLORE,DELANO,GROS, GROSSE-KUNSTLEVE,JIANG,KUSZEWSKI,NILGES, PANNU,READ,RICE,SIMONSON,WARREN - refinement

NMRView, Johnson, One Moon Scientific - structure solution

NMR spectrometers:

  • VARIAN UNITYINOVA 750 MHz

Related Database Links:

PDB
DBJ BAB22086 BAB25616 BAB27066 BAB28217 BAB79475
EMBL CAG47008 CAG47044
GB AAA36472 AAC48755 AAH05354 AAH05920 AAH07573
REF NP_000995 NP_001078905 NP_001180505 NP_001231795 NP_001270094
SP P05387 P19943 P42899 P99027 Q6X9Z5
TPG DAA13527
AlphaFold P05387 P19943 P42899 P99027 Q6X9Z5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks