BMRB Entry 15990
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15990
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Hong, Eunmi; Doucleff, Michaeleen; Wemmer, David. "Structure of the RNA Polymerase Core-Binding Domain of sigma(54) Reveals a Likely Conformational Fracture Point." J. Mol. Biol. 390, 70-82 (2009).
PubMed: 19426742
Assembly members:
RNAP, polymer, 76 residues, Formula weight is not available
Natural source: Common Name: Aquifex aeolicus Taxonomy ID: 63363 Superkingdom: Bacteria Kingdom: not available Genus/species: Aquifex aeolicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21b3-2
Entity Sequences (FASTA):
RNAP: KETVPYQIPYTPSELEELQQ
NIKLELEGKEQELALELLNY
LNEKGFLSKSVEEISDVLRC
SVEELEKVRQKVLRLE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 254 |
| 15N chemical shifts | 72 |
| 1H chemical shifts | 558 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | subunit 1 | 1 |
Entities:
Entity 1, subunit 1 76 residues - Formula weight is not available
| 1 | LYS | GLU | THR | VAL | PRO | TYR | GLN | ILE | PRO | TYR | ||||
| 2 | THR | PRO | SER | GLU | LEU | GLU | GLU | LEU | GLN | GLN | ||||
| 3 | ASN | ILE | LYS | LEU | GLU | LEU | GLU | GLY | LYS | GLU | ||||
| 4 | GLN | GLU | LEU | ALA | LEU | GLU | LEU | LEU | ASN | TYR | ||||
| 5 | LEU | ASN | GLU | LYS | GLY | PHE | LEU | SER | LYS | SER | ||||
| 6 | VAL | GLU | GLU | ILE | SER | ASP | VAL | LEU | ARG | CYS | ||||
| 7 | SER | VAL | GLU | GLU | LEU | GLU | LYS | VAL | ARG | GLN | ||||
| 8 | LYS | VAL | LEU | ARG | LEU | GLU |
Samples:
sample_1: RNAP, [U-100% 13C; U-100% 15N], 1-1.5 mM; RNAP, [U-100% 15N], 1-1.5 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker DRX 800 MHz
- Bruker DRX 900 MHz
- Bruker DRX 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks