BMRB Entry 15987

Name: NMR structure of plasmid copy control protein ORF56 from sulfolobus islandicus
Published: 2009-10-16

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PDB ID: 2k9i
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15987
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of plasmid copy control protein ORF56 from sulfolobus islandicus

Deposition date:

2008-10-15

Original release date:

2009-10-16

Authors:

Weininger, Ulrich; Balbach, Jochen

Citation:

Citation: Weininger, Ulrich; Zeeb, Markus; Neumann, Piotr; Low, Christian; Stubbs, Milton; Lipps, Georg; Balbach, Jochen. "Structure-based stability analysis of an extremely stable dimeric DNA binding protein from Sulfolobus islandicus." Biochemistry 48, 10030-10037 (2009).
PubMed: 19788170

Assembly members:

Assembly members:
ORF56, polymer, 55 residues, 149.207 Da.

Natural source:

Natural source: Common Name: Sulfolobus islandicus Taxonomy ID: 43080 Superkingdom: Archaea Kingdom: not available Genus/species: Sulfolobus islandicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28c

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ORF56: GRPYKLLNGIKLGVYIPQEW HDRLMEIAKEKNLTLSDVCR LAIKEYLDNHDKQKK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	427

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	METHIONINE, chain 1	1
2	METHIONINE, chain 2	1

Entities:

Entity 1, METHIONINE, chain 1 55 residues - 149.207 Da.

1

GLY

ARG

PRO

TYR

LYS

LEU

ASN

GLY

ILE

2

LYS

LEU

GLY

VAL

TYR

ILE

PRO

GLN

GLU

TRP

3

HIS

ASP

ARG

LEU

MET

GLU

ILE

ALA

LYS

GLU

4

LYS

ASN

LEU

THR

LEU

SER

ASP

VAL

CYS

ARG

5

LEU

ALA

ILE

LYS

GLU

TYR

LEU

ASP

ASN

HIS

6

ASP

LYS

GLN

LYS

Samples:

sample_1: entity_2, [U-13C; U-15N], 1 mM; entity_2 2 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

NMR spectrometers:

Bruker Avance 700 MHz
Bruker DRX 400 MHz