BMRB Entry 15971

Name: SOLUTION STRUCTURE OF THE LSM DOMAIN OF DROSOPHILA MELANOGASTER TRAL (TRAILER HITCH)
Published: 2009-03-18

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15971

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SOLUTION STRUCTURE OF THE LSM DOMAIN OF DROSOPHILA MELANOGASTER TRAL (TRAILER HITCH)

Deposition date:

2008-10-02

Original release date:

2009-03-18

Authors:

Tritschler, F.; Coles, M.; Truffault, V.

Citation:

Citation: Tritschler, F.; Eulalio, A.; Helms, S.; Weichenrieder, O.; Schmidt, S.; Izaurralde, E.; Truffault, V.. "Similar Modes of Interaction Enables Trailer Hitch and Edc3 to Associate with Dcp1 and Me31B in Distinct Protein Complexes" Mol. Cell. Biol. 28, 6695-6708 (2008).
PubMed: 18765641

Assembly members:

Assembly members:
DANIO_RERIO_RAP55, polymer, 95 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Zebra fish Taxonomy ID: 7955 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Danio rerio

Experimental source:

Experimental source: Production method: recombinant technology Host organism: CELL-FREE SYNTHESIS Vector: PEU-HIS

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DANIO_RERIO_RAP55: GHHHHHHLEDPSGGTPYIGS KISLISKAEIRYEGILYTID TENSTVALAKVRSFGTEDRP TDRPIAPRDETFEYIIFRGS DIKDLTVCEPPKPIM

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	346
15N chemical shifts	80
1H chemical shifts	581

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DANIO RERIO RAP55	1

Entities:

Entity 1, DANIO RERIO RAP55 95 residues - Formula weight is not available

1

GLY

HIS

LEU

GLU

ASP

2

PRO

SER

GLY

THR

PRO

TYR

ILE

GLY

SER

3

LYS

ILE

SER

LEU

ILE

SER

LYS

ALA

GLU

ILE

4

ARG

TYR

GLU

GLY

ILE

LEU

TYR

THR

ILE

ASP

5

THR

GLU

ASN

SER

THR

VAL

ALA

LEU

ALA

LYS

6

VAL

ARG

SER

PHE

GLY

THR

GLU

ASP

ARG

PRO

7

THR

ASP

ARG

PRO

ILE

ALA

PRO

ARG

ASP

GLU

8

THR

PHE

GLU

TYR

ILE

PHE

ARG

GLY

SER

9

ASP

ILE

LYS

ASP

LEU

THR

VAL

CYS

GLU

PRO

10

PRO

LYS

PRO

ILE

MET

Samples:

sample_1: DANIO RERIO RAP55, [U-13C; U-15N], 0.8 mM; NACL 100 mM; BIS- TRIS 10 mM; DTT 5 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1.0 atm; temperature: 289.0 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D_15N-SEPARATED_NOESY	sample_1	isotropic	sample_conditions_1
3D_13C-SEPARATED_NOESY	sample_1	isotropic	sample_conditions_1
HNCO	sample_1	isotropic	sample_conditions_1
HNCACB	sample_1	isotropic	sample_conditions_1
CCCONH	sample_1	isotropic	sample_conditions_1
HCCCONH	sample_1	isotropic	sample_conditions_1
CBCACONH	sample_1	isotropic	sample_conditions_1
HBHACONH	sample_1	isotropic	sample_conditions_1
HCH-TOCSY	sample_1	isotropic	sample_conditions_1
CCH- TOCSY	sample_1	isotropic	sample_conditions_1

Software:

X-PLOR v2.9.4, BRUNGER - refinement

SPARKY, Goddard - structure solution

NMR spectrometers:

VARIAN UNITYINOVA 600 MHz

PDB	2FB7 2VXF
DBJ	BAB55259 BAC99045 BAC99046 BAF36055 BAG10232
EMBL	CAG31566 CAH93252 CDQ65324 CDQ79842 CDQ84551
GB	AAH16842 AAH31521 AAH42251 AAH54659 AAH55387
REF	NP_001012796 NP_001029826 NP_001079434 NP_001087507 NP_001107565
SP	A0A8M2 Q3MHF8 Q5R4R4 Q6NVR8 Q8AVJ2
TPG	DAA19959
AlphaFold	A0A8M2 Q3MHF8 Q5R4R4 Q6NVR8 Q8AVJ2