BMRB Entry 15970
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15970
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NMR-STAR v3 text file.
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Citation: Tritschler, F.; Eulalio, A.; Helms, S.; Weichenrieder, O.; Schmidt, S.; Izaurralde, E.; Truffault, V.. "Similar Modes of Interaction Enable Trailer Hitch and Edc3 to Associate with Dcp1 and Me31B in Distinct Protein Complexes" Mol. Cell. Biol 28, 6695-6708 (2008).
PubMed: 18765641
Assembly members:
TRAL-TRAILER_HITCH, polymer, 88 residues, Formula weight is not available
Natural source: Common Name: FRUIT FLY Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: DROSOPHILA MELANOGASTER
Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI Vector: PETM60
Entity Sequences (FASTA):
TRAL-TRAILER_HITCH: GAMAMSGGLPELGSKISLIS
KADIRYEGRLYTVDPQECTI
ALSSVRSFGTEDRDTQFQIA
PQSQIYDYILFRGSDIKDIR
VVNNHTLP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 327 |
| 15N chemical shifts | 71 |
| 1H chemical shifts | 540 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TRAL-TRAILER HITCH | 1 |
Entities:
Entity 1, TRAL-TRAILER HITCH 88 residues - Formula weight is not available
| 1 | GLY | ALA | MET | ALA | MET | SER | GLY | GLY | LEU | PRO | ||||
| 2 | GLU | LEU | GLY | SER | LYS | ILE | SER | LEU | ILE | SER | ||||
| 3 | LYS | ALA | ASP | ILE | ARG | TYR | GLU | GLY | ARG | LEU | ||||
| 4 | TYR | THR | VAL | ASP | PRO | GLN | GLU | CYS | THR | ILE | ||||
| 5 | ALA | LEU | SER | SER | VAL | ARG | SER | PHE | GLY | THR | ||||
| 6 | GLU | ASP | ARG | ASP | THR | GLN | PHE | GLN | ILE | ALA | ||||
| 7 | PRO | GLN | SER | GLN | ILE | TYR | ASP | TYR | ILE | LEU | ||||
| 8 | PHE | ARG | GLY | SER | ASP | ILE | LYS | ASP | ILE | ARG | ||||
| 9 | VAL | VAL | ASN | ASN | HIS | THR | LEU | PRO |
Samples:
sample_1: TRAL-TRAILER HITCH, [U-15N], 0.6 mM; DTT 1 mM
sample_2: TRAL-TRAILER HITCH, [U-15N; U-13C], 0.6 mM; DTT 1 mM
sample_conditions_1: ionic strength: 150 mM; pH: 7.2; pressure: 1.0 atm; temperature: 298.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCACO | sample_1 | isotropic | sample_conditions_1 |
| HNCO | sample_1 | isotropic | sample_conditions_1 |
| HNCACB | sample_1 | isotropic | sample_conditions_1 |
| CBCACONH | sample_1 | isotropic | sample_conditions_1 |
| HNHA | sample_1 | isotropic | sample_conditions_1 |
| HNHB | sample_1 | isotropic | sample_conditions_1 |
| CCH- COSY | sample_1 | isotropic | sample_conditions_1 |
| CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| HNH-NOESY | sample_1 | isotropic | sample_conditions_1 |
| NNH-NOESY | sample_1 | isotropic | sample_conditions_1 |
| CNH-NOESY | sample_1 | isotropic | sample_conditions_1 |
| HCH- NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR v2.9.4, BRUNGER - refinement
SPARKY, Goddard - structure solution
NMR spectrometers:
- BRUKER OTHER 900 MHz
Related Database Links:
| PDB | |
| GB | AAF49905 AAL39211 AGB94462 AGB94463 AGB94464 |
| REF | NP_001261769 NP_001261770 NP_001261771 NP_001261772 NP_001261773 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks