BMRB Entry 15968

Title:
Backbone chemical shift assignments for the UBA domain of Swa2p
Deposition date:
2008-09-29
Original release date:
2008-11-05
Authors:
Matta-Camacho, Edna; Kozlov, Guennadi; Trempe, Jean-Francois; Gehring, Kalle
Citation:

Citation: Matta-Camacho, Edna; Kozlov, Guennadi; Trempe, Jean-Francois; Gehring, Kalle. "Atypical binding of the Swa2p UBA domain to ubiquitin"  J. Mol. Biol. 386, 569-577 (2009).
PubMed: 18948116

Assembly members:

Assembly members:
Swa2p, polymer, 52 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P1

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts93
15N chemical shifts46
1H chemical shifts46

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1polypeptide chain1

Entities:

Entity 1, polypeptide chain 52 residues - Formula weight is not available

1   GLYPROLEUGLYSERALALEUVALASPGLU
2   VALLYSASPMETGLUILEALAARGLEUMET
3   SERLEUGLYLEUSERILEGLUGLUALATHR
4   GLUPHETYRGLUASNASPVALTHRTYRGLU
5   ARGTYRLEUGLUILELEULYSSERLYSGLN
6   LYSGLU

Samples:

sample_1: Swa2p, [U-13C; U-15N], 0.5-1.0 mM; HEPES 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

XEASY, Bartels et al. - data analysis

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ GAA22540
EMBL CAY78820
GB AAB64756 AHY75293 AJP38020 AJU58143 AJU58836
REF NP_010606
SP Q06677
TPG DAA12161
AlphaFold Q06677

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks