BMRB Entry 15968

Name: Backbone chemical shift assignments for the UBA domain of Swa2p
Published: 2008-11-05

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15968

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone chemical shift assignments for the UBA domain of Swa2p

Deposition date:

2008-09-29

Original release date:

2008-11-05

Authors:

Matta-Camacho, Edna; Kozlov, Guennadi; Trempe, Jean-Francois; Gehring, Kalle

Citation:

Citation: Matta-Camacho, Edna; Kozlov, Guennadi; Trempe, Jean-Francois; Gehring, Kalle. "Atypical binding of the Swa2p UBA domain to ubiquitin" J. Mol. Biol. 386, 569-577 (2009).
PubMed: 18948116

Assembly members:

Assembly members:
Swa2p, polymer, 52 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Swa2p: GPLGSALVDEVKDMEIARLM SLGLSIEEATEFYENDVTYE RYLEILKSKQKE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	93
15N chemical shifts	46
1H chemical shifts	46

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	polypeptide chain	1

Entities:

Entity 1, polypeptide chain 52 residues - Formula weight is not available

1

GLY

PRO

LEU

GLY

SER

ALA

LEU

VAL

ASP

GLU

2

VAL

LYS

ASP

MET

GLU

ILE

ALA

ARG

LEU

MET

3

SER

LEU

GLY

LEU

SER

ILE

GLU

ALA

THR

4

GLU

PHE

TYR

GLU

ASN

ASP

VAL

THR

TYR

GLU

5

ARG

TYR

LEU

GLU

ILE

LEU

LYS

SER

LYS

GLN

6

LYS

GLU

Samples:

sample_1: Swa2p, [U-13C; U-15N], 0.5-1.0 mM; HEPES 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

XEASY, Bartels et al. - data analysis

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 600 MHz

PDB	1PGY
DBJ	GAA22540
EMBL	CAY78820
GB	AAB64756 AHY75293 AJP38020 AJU58143 AJU58836
REF	NP_010606
SP	Q06677
TPG	DAA12161
AlphaFold	Q06677