BMRB Entry 15965

Assembly:

Entities:

Entity 2, entity_2 37 residues - 4115.06 Da.

Residues 252-284 are encoded by exon 8 and 9d or rat alpha tropomyosin (TPM1 gene). Residues 248-251, GCGG were added to allow disulfide cross linking.

Entity 1, entity_1 38 residues - 4331.956 Da.

Residue 0 is a glycine group replacing a native acetyl group residue 1-19 are the first 19 residues of a rat non-muscle low molecular weight tropomyosin encoded by exon 1b (TPM1 gene). 0 is a glycine residue that substitutes for the native acetyl group. Residues 20-37 are the last 18 residues of the yeast transcription factor GCN4.

Samples:

sample_1: TM1b(1-14)Zip, [U-99% 13C; U-99% 15N], 1 ± 0.1 mM; TM9d(252-284) 1 ± 0.1 mM; NaCl 100 mM; sodium phosphate 10 mM

sample_2: TM1b(1-19)Zip, [U-15N], 1 ± 0.1 mM; TM9d(252-284) 1 ± 0.1 mM; NaCl 100 mM; sodium phosphate 10 mM

sample_3: TM1b(1-19)Zip, [U-99% 13C; U-99% 15N], 0.5 ± 0.05 mM; TM1b(1-19)Zip 0.5 ± 0.05 mM; TM9d(252-284) 1 ± 0.1 mM; NaCl 100 mM; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 0.14 M; pH: 6.4; pressure: 1 atm; temperature: 293 K

Experiments:

Software:

AutoStruct v1.12, Huang, Tejero, Powers and Montelione - structure solution

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - geometry optimization

SPARKY v3.111, Goddard - peak picking

NMRPipe v1.1, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

• Varian INOVA 600 MHz
• Bruker Biospin 800 MHz