BMRB Entry 15946

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15946
MolProbity Validation Chart

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Title:
Solution structure of a paralog-specific Mena binder by NMR
Deposition date:
2008-09-09
Original release date:
2009-04-04
Authors:
Link, Nina; Hunke, Cornelia; Eichler, Jutta; Bayer, Peter
Citation:

Citation: Link, Nina; Hunke, Cornelia; Mueller, Jonathan; Eichler, Jutta; Bayer, Peter. "The solution structure of pGolemi, a high affinity Mena EVH1 binding miniature protein, suggests explanations for paralog-specific binding to Ena/VASP homology (EVH) 1 domains"  Biol. Chem. 390, 417-26 (2009).
PubMed: 19284291

Assembly members:

Assembly members:
pGolemi, polymer, 30 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
pGolemi: PFPPTPPGEEAPVEDLIRFY NDLQQYLNVV

Data sets:
Data typeCount
1H chemical shifts206

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1pGolemi1

Entities:

Entity 1, pGolemi 30 residues - Formula weight is not available

1   PROPHEPROPROTHRPROPROGLYGLUGLU
2   ALAPROVALGLUASPLEUILEARGPHETYR
3   ASNASPLEUGLNGLNTYRLEUASNVALVAL

Samples:

sample_1: pGolemi 1 mM; H2O 90%; D2O 10%; phosphate buffer 45 mM

sample_conditions_1: ionic strength: 45 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
COSYsample_1isotropicsample_conditions_1
TOCSYsample_1isotropicsample_conditions_1
NOESYsample_1isotropicsample_conditions_1

Software:

AUREMOL v2.0, Bruker Biospin - chemical shift assignment, peak picking

X-PLOR NIH v2.18, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz

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