BMRB Entry 15939

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15939
MolProbity Validation Chart

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Title:
Solution Structure of FOXO3a Forkhead domain
Deposition date:
2008-09-02
Original release date:
2009-02-16
Authors:
Wang, Feng; Marshall, Christopher; Li, Guang-Yao; Plevin, Michael; Ikura, Mitsuhiko
Citation:

Citation: Wang, Feng; Marshall, Christopher; Yamamoto, Kazuo; Li, Guang-Yao; Plevin, Michael; You, Han; Mak, Tak; Ikura, Mitsuhiko. "Biochemical and structural characterization of an intramolecular interaction in FOXO3a and its binding with p53"  J. Mol. Biol. 384, 590-603 (2008).
PubMed: 18824006

Assembly members:

Assembly members:
FOXO3a, polymer, 103 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX4T-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FOXO3a: GSSSRRNAWGNLSYADLITR AIESSPDKRLTLSQIYEWMV RCVPYFKDKGDSNSSAGWKN SIRHNLSLHSRFMRVQNEGT GKSSWWIINPDGGKSGKAPR RRA

Data sets:
Data typeCount
13C chemical shifts374
15N chemical shifts93
1H chemical shifts593

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GLYCINE1

Entities:

Entity 1, GLYCINE 103 residues - Formula weight is not available

1 and 2 are residues from vector. 3-103 are region 151-250 of human FOXO3a.

1   GLYSERSERSERARGARGASNALATRPGLY
2   ASNLEUSERTYRALAASPLEUILETHRARG
3   ALAILEGLUSERSERPROASPLYSARGLEU
4   THRLEUSERGLNILETYRGLUTRPMETVAL
5   ARGCYSVALPROTYRPHELYSASPLYSGLY
6   ASPSERASNSERSERALAGLYTRPLYSASN
7   SERILEARGHISASNLEUSERLEUHISSER
8   ARGPHEMETARGVALGLNASNGLUGLYTHR
9   GLYLYSSERSERTRPTRPILEILEASNPRO
10   ASPGLYGLYLYSSERGLYLYSALAPROARG
11   ARGARGALA

Samples:

sample_1: sodium phosphate 20 mM; potassium chloride 50 mM; DTT 2 mM; sodium azide 0.5 mM

Sample_2: sodium phosphate 20 mM; potassium chloride 50 mM; DTT 2 mM; sodium azide 0.5 mM

sample_conditions_1: ionic strength: 0.08 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYSample_2isotropicsample_conditions_1
2D 1H-13C HSQCSample_2isotropicsample_conditions_1
3D HCCH-TOCSYSample_2isotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment, peak picking

TALOS, Cornilescu, Delaglio and Bax - data analysis

ProcheckNMR, Laskowski and MacArthur - data analysis

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

PDB
DBJ BAC33049 BAG74316 BAJ21194
GB AAC39592 AAD42107 AAH19532 AAH20227 AAH21224
REF NP_001099865 NP_001129431 NP_001193012 NP_001254818 NP_001446
SP O43524 Q9WVH4
TPG DAA26278
AlphaFold O43524 Q9WVH4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks