BMRB Entry 15934

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15934

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for M-crystallin (calcium bound form) in presence of 6 M Gdn-Hcl
Deposition date:
2008-08-30
Original release date:
2009-03-23
Authors:
Barnwal, Ravi Pratap
Citation:

Citation: Barnwal, Ravi; Agarwal, Geetika; Sharma, Yogendra; Chary, Kandala. "Complete backbone assignment of a Ca2+-binding protein of the betagamma-crystallin superfamily from Methanosarcina acetivorans, at two denaturant concentrations"  Biomol. NMR Assignments 3, 107-110 (2009).
PubMed: 19636958

Assembly members:

Assembly members:
M-crystallin_in_6M_Gdn-HCl, polymer, 85 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
M-crystallin_in_6M_Gdn-HCl: MNAAEVIVYEHVNFGGKSFD ATSDQPGAGDNLNDKISSIK VKSGTWRFYEYINYGGRYWD LGPGEYSSVESAGIPDNSIS SFRQI

Data sets:
Data typeCount
15N chemical shifts80
1H chemical shifts80

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11
2calcium ion2

Entities:

Entity 1, subunit 1 85 residues - Formula weight is not available

1   METASNALAALAGLUVALILEVALTYRGLU
2   HISVALASNPHEGLYGLYLYSSERPHEASP
3   ALATHRSERASPGLNPROGLYALAGLYASP
4   ASNLEUASNASPLYSILESERSERILELYS
5   VALLYSSERGLYTHRTRPARGPHETYRGLU
6   TYRILEASNTYRGLYGLYARGTYRTRPASP
7   LEUGLYPROGLYGLUTYRSERSERVALGLU
8   SERALAGLYILEPROASPASNSERILESER
9   SERPHEARGGLNILE

Entity 2, calcium ion - Ca - 40.078 Da.

1   CA

Samples:

sample_1: M-crystallin in 6M Gdn-HCl, [U-99% 13C; U-99% 15N], 1.3 mM; Calcium 6-10 mM; Gdn-Hcl 6000 mM

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15918
PDB
GB AAM05909
REF WP_048065429

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks