BMRB Entry 15911

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15911

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Title:
NMR backbone assignments of the periplasmic loop P2 of the MalF subunit of the maltose ATP binding cassette transporter
Deposition date:
2008-08-07
Original release date:
2008-10-02
Authors:
Jacso, Tomas; Grote, Mathias; Schmieder, Peter; Schneider, Erwin; Reif, Bernd
Citation:

Citation: Jacso, Tomas; Grote, Mathias; Daus, Martin; Schmieder, Peter; Keller, Sandro; Schneider, Erwin; Reif, Bernd. "The periplasmic loop P2 of the MalF subunit of the maltose ATP binding cassette transporter is sufficient to bind the maltose binding protein MalE"  Biochemistry 48, 2216-2225 (2009).
PubMed: 19159328

Assembly members:

Assembly members:
MalF-P2, polymer, 184 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MalF-P2: MNYSSTNQLTFERAQEVLLD RSWQAGKTYNFGLYPAGDEW QLALSDGETGKNYLSDAFKF GGEQKLQLKETTAQPEGERA NLRVITQNRQALSDITAILP DGNKVMMSSLRQFSGTQPLY TLDGDGTLTNNQSGVKYRPN NQIGFYQSITADGNWGDEKL SPGYTVTTGWKNFTRVFTDE GIQK

Data sets:
Data typeCount
13C chemical shifts329
15N chemical shifts157
1H chemical shifts157

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MalF-P21

Entities:

Entity 1, MalF-P2 184 residues - Formula weight is not available

The second residue in MalF-P2 is residue N93 in MalF

1   METASNTYRSERSERTHRASNGLNLEUTHR
2   PHEGLUARGALAGLNGLUVALLEULEUASP
3   ARGSERTRPGLNALAGLYLYSTHRTYRASN
4   PHEGLYLEUTYRPROALAGLYASPGLUTRP
5   GLNLEUALALEUSERASPGLYGLUTHRGLY
6   LYSASNTYRLEUSERASPALAPHELYSPHE
7   GLYGLYGLUGLNLYSLEUGLNLEULYSGLU
8   THRTHRALAGLNPROGLUGLYGLUARGALA
9   ASNLEUARGVALILETHRGLNASNARGGLN
10   ALALEUSERASPILETHRALAILELEUPRO
11   ASPGLYASNLYSVALMETMETSERSERLEU
12   ARGGLNPHESERGLYTHRGLNPROLEUTYR
13   THRLEUASPGLYASPGLYTHRLEUTHRASN
14   ASNGLNSERGLYVALLYSTYRARGPROASN
15   ASNGLNILEGLYPHETYRGLNSERILETHR
16   ALAASPGLYASNTRPGLYASPGLULYSLEU
17   SERPROGLYTYRTHRVALTHRTHRGLYTRP
18   LYSASNPHETHRARGVALPHETHRASPGLU
19   GLYILEGLNLYS

Samples:

sample_1: MalF-P2, [U-100% 13C; U-100% 15N; 80% 2H], 1.0 ± 0.1 mM; H2O 90%; D2O, [U-100% 2H], 10%; phosphate 20 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CcpNMR, CCPN - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAB38439 BAE78035 BAG79848 BAI57430 BAJ45745
EMBL CAP78493 CAQ34382 CAR01011 CAR05668 CAR10710
GB AAB59055 AAC43127 AAC77003 AAG59232 AAN45593
REF NP_313043 NP_418457 NP_709886 WP_001295280 WP_001296633
SP P02916 Q7A937 Q83P81 Q8FB38
AlphaFold P02916 Q7A937 Q83P81 Q8FB38

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks