BMRB Entry 15900

Title:
MMP23, a metalloprotease with a toxin-like potassium channel blocking domain and immunomodulatory role with relevance in prostate cancer
Deposition date:
2008-07-31
Original release date:
2009-12-11
Authors:
Khoo, Keith; Feng, Zhiping
Citation:

Citation: Rangaraju, Srikant; Khoo, Keith; Feng, Zhi-Ping; Crossley, George; Nugent, Daniel; Khaytin, Ilya; Chi, Victor; Pham, Cory; Calabresi, Peter; Pennington, Michael; Norton, Raymond; Chandy, K. George. "Potassium channel modulation by a toxin domain in matrix metalloprotease 23."  J. Biol. Chem. 285, 9124-9136 (2010).
PubMed: 19965868

Assembly members:

Assembly members:
MMP23-BgK, polymer, 37 residues, 4442.344 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MMP23-BgK: YGCLDRIFVCTSWARKGFCD VRQRLMKRLCPRSCDFC

Data sets:
Data typeCount
1H chemical shifts270

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MMP23-BgK1

Entities:

Entity 1, MMP23-BgK 37 residues - 4442.344 Da.

1   TYRGLYCYSLEUASPARGILEPHEVALCYS
2   THRSERTRPALAARGLYSGLYPHECYSASP
3   VALARGGLNARGLEUMETLYSARGLEUCYS
4   PROARGSERCYSASPPHECYS

Samples:

94%H2O_6%D2O: MMP23-BgK 2.26 mM; H2O 94%; D2O, [U-100% 2H], 6%

100%D2O: MMP23-BgK 2.2-2.3 mM; D2O, [U-100% 2H], 100%

278K_pH5.00: pH: 5.00; pressure: 1 atm; temperature: 278 K

293K_pH5.00: pH: 5.00; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSY94%H2O_6%D2Oisotropic278K_pH5.00
2D 1H-1H NOESY94%H2O_6%D2Oisotropic278K_pH5.00
2D DQF-COSY94%H2O_6%D2Oisotropic293K_pH5.00
2D 1H-13C HSQC94%H2O_6%D2Oisotropic293K_pH5.00
2D 1H-15N HSQC94%H2O_6%D2Oisotropic293K_pH5.00
2D 1H-1H TOCSY100%D2Oisotropic278K_pH5.00
2D 1H-1H NOESY100%D2Oisotropic278K_pH5.00
2D 1H-1H TOCSY94%H2O_6%D2Oisotropic293K_pH5.00
2D 1H-1H NOESY94%H2O_6%D2Oisotropic293K_pH5.00

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

XEASY vv1.3.13, Bartels et al. - chemical shift assignment, peak picking

TOPSPIN vv1.3, Bruker Biospin - collection, processing

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

TALOS, Cornilescu, Delaglio and Bax - geometry optimization

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker Avance 500 MHz

Related Database Links:

PDB
DBJ BAA24832 BAE27223
GB AAC34886 AAH86586 AAI07358 AAI07359 EDL15021
REF NP_036115 NP_446058 XP_006538942 XP_006538943 XP_006538944
SP O88272 O88676
AlphaFold O88272 O88676