BMRB Entry 15882

Name: 1H, 15N, 13C backbone assignment of the SPF45 UHM domain
Published: 2008-10-01

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N, 13C backbone assignment of the SPF45 UHM domain

Deposition date:

2008-07-22

Original release date:

2008-10-01

Authors:

Corsini, Lorenzo; Sattler, Michael

Citation:

Citation: Corsini, Lorenzo; Bonnal, Sophie; Basquin, Jerome; Hothorn, Michael; Scheffzek, Klaus; Valcarcel, Juan; Sattler, Michael. "U2AF-homology motif interactions are required for alternative splicing regulation by SPF45." Nat. Struct. Biol. 14, 620-629 (2007).
PubMed: 17589525

Assembly members:

Assembly members:
SPF45, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET9d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SPF45: GAMKCPTKVVLLRNMVGAGE VDEDLEVETKEECEKYGKVG KCVIFEIPGAPDDEAVRIFL EFERVESAIKAVVDLNGRYF GGRVVKACFYNLDKFRVLDL AEQV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	196
1H chemical shifts	96
15N chemical shifts	96

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SPF45	1

Entities:

Entity 1, SPF45 104 residues - Formula weight is not available

1

GLY

ALA

MET

LYS

CYS

PRO

THR

LYS

VAL

2

LEU

ARG

ASN

MET

VAL

GLY

ALA

GLY

GLU

3

VAL

ASP

GLU

ASP

LEU

GLU

VAL

GLU

THR

LYS

4

GLU

CYS

GLU

LYS

TYR

GLY

LYS

VAL

GLY

5

LYS

CYS

VAL

ILE

PHE

GLU

ILE

PRO

GLY

ALA

6

PRO

ASP

GLU

ALA

VAL

ARG

ILE

PHE

LEU

7

GLU

PHE

GLU

ARG

VAL

GLU

SER

ALA

ILE

LYS

8

ALA

VAL

ASP

LEU

ASN

GLY

ARG

TYR

PHE

9

GLY

ARG

VAL

LYS

ALA

CYS

PHE

TYR

10

ASN

LEU

ASP

LYS

PHE

ARG

VAL

LEU

ASP

LEU

11

ALA

GLU

GLN

VAL

Samples:

sample_1: SPF45, [U-100% 13C; U-100% 15N], 0.5 ± 0.1 mM

sample_conditions_1: temperature: 300 K; pH: 7.00; pressure: 1 atm; ionic strength: 0.2 M

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRView v5.0.2, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker DRX 500 MHz

PDB	2PE8 2PEH
DBJ	BAE91606 BAG37457 BAI47074 BAK63294
GB	AAC64085 AAH07871 AAH09064 AAH34896 AAH39322
REF	NP_001013076 NP_001094553 NP_001139019 NP_001244661 NP_001267436
SP	Q8JZX4 Q96I25
TPG	DAA16000 DAA23631
AlphaFold	Q8JZX4 Q96I25