BMRB Entry 15880

Name: Structure of Rab11-FIP2 C-terminal Coiled-coil Domain
Published: 2009-02-13

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PDB ID: 2k6s
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15880
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of Rab11-FIP2 C-terminal Coiled-coil Domain

Deposition date:

2008-07-18

Original release date:

2009-02-13

Authors:

Wei, Jie; Liu, Yuqi; Baleja, James

Citation:

Citation: Wei, Jie; Liu, Yuqi; Bose, Kakoli; Henry, Gillian; Baleja, Jim. "Disorder and structure in the Rab11 binding domain of Rab11 family interacting protein 2." Biochemistry 48, 549-557 (2009).
PubMed: 19119858

Assembly members:

Assembly members:
Rab11-FIP2, polymer, 41 residues, 5004.829 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Human sapien

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET19b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rab11-FIP2: GSLTYEEVLQELVKHKELLR RKDTHIRELEDYIDNLLVRV M

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	60
15N chemical shifts	35
1H chemical shifts	299

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2	1

Entities:

Entity 1, entity_1 41 residues - 5004.829 Da.

1

GLY

SER

LEU

THR

TYR

GLU

VAL

LEU

GLN

2

GLU

LEU

VAL

LYS

HIS

LYS

GLU

LEU

ARG

3

ARG

LYS

ASP

THR

HIS

ILE

ARG

GLU

LEU

GLU

4

ASP

TYR

ILE

ASP

ASN

LEU

VAL

ARG

VAL

5

MET

Samples:

sample_1: entity 1 mM; DSS 20 uM; sodium phosphate 20 mM; D2O, [U-100% 2H], 100%

sample_2: entity, [U-100% 15N], 1.5 mM; DSS 20 uM; sodium phosphate 20 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_3: entity, [U-100% 13C; U-100% 15N], 0.8 mM; DSS 20 uM; sodium phosphate 20 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 0.08 M; pH: 7.2; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_3	isotropic	sample_conditions_1
3D HNCA	sample_3	isotropic	sample_conditions_1
3D HNCACB	sample_3	isotropic	sample_conditions_1
3D HN(CO)CA	sample_3	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_3	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_3	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1

Software:

xwinnmr v3.5, Bruker Biospin - collection

TOPSPIN v1.3, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

PDB	2GZD 2GZH 2K6S 3TSO 4C4P
DBJ	BAA76785 BAE32923 BAG09909
GB	AAH75073 AAH75074 AAH89010 AAI39381 AAI39420
REF	NP_001028344 NP_001100917 NP_001157839 NP_001179700 NP_055719
SP	G3XA57 Q7L804
TPG	DAA14748
AlphaFold	G3XA57 Q7L804