BMRB Entry 15871

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15871

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Title:
Solution Structure of XACb0070 from Xanthonomas axonopodis pv citri
Deposition date:
2008-07-10
Original release date:
2009-10-20
Authors:
Gallo, Mariana; Cicero, Daniel; Amata, Irene; Eliseo, Tommaso; Paci, Maurizio; Spisni, Alberto; Ferrari, Elena; Pertinhez, Thelma; Farah, Chuck
Citation:

Citation: Gallo, Mariana; Ferrari, Elena; Eliseo, Tommaso; Amata, Irene; Pertinhez, Thelma; Katsuyama, Angela; Paci, Maurizio; Farah, Chuck; Spisni, Alberto; Cicero, Daniel. "A new member of the ribbon-helix-helix transcription factor superfamily from the plant pathogen Xanthomonas axonopodis pv.citri."  J. Struct. Biol. 170, 21-31 (2010).
PubMed: 20060909

Assembly members:

Assembly members:
XACb0070, polymer, 79 residues, 8809 Da.

Natural source:

Natural source:   Common Name: Xanthomonas axonopodis   Taxonomy ID: 92829   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Xanthomonas axonopodis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
XACb0070: MNTVRWNIAVSPDVDQSVRM FIAAQGGGRKGDLSRFIEDA VRAYLFERAVEQAKAATVGM GETELNDLIDEAVQWAREH

Data sets:
Data typeCount
13C chemical shifts337
15N chemical shifts51
1H chemical shifts495

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11
2subunit 21

Entities:

Entity 1, subunit 1 79 residues - 8809 Da.

1   METASNTHRVALARGTRPASNILEALAVAL
2   SERPROASPVALASPGLNSERVALARGMET
3   PHEILEALAALAGLNGLYGLYGLYARGLYS
4   GLYASPLEUSERARGPHEILEGLUASPALA
5   VALARGALATYRLEUPHEGLUARGALAVAL
6   GLUGLNALALYSALAALATHRVALGLYMET
7   GLYGLUTHRGLULEUASNASPLEUILEASP
8   GLUALAVALGLNTRPALAARGGLUHIS

Samples:

sample_1: sodium phosphate 40 mM; sodium chloride 50 mM; sodium azide 0.05%; XACb0070 0.3 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_2: sodium phosphate 40 mM; sodium chloride 300 mM; sodium azide 0.05%; XACb0070 0.5 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_3: sodium phosphate 40 mM; sodium chloride 300 mM; sodium azide 0.05%; XACb0070 0.5 mM; D2O, [U-100% 2H], 100%

sample_conditions_1: ionic strength: 170 mM; pH: 6.7; pressure: 1 atm; temperature: 313 K

sample_conditions_2: ionic strength: 420 mM; pH: 9.00; pressure: 1 atm; temperature: 308.0 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_2
2D 1H-13C HSQCsample_3isotropicsample_conditions_2
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_2
3D HNCOsample_2isotropicsample_conditions_2
3D HNCAsample_2isotropicsample_conditions_2
3D HBHA(CO)NHsample_2isotropicsample_conditions_2
2D 1H-1H NOESYsample_3isotropicsample_conditions_2
HetNOEsample_2isotropicsample_conditions_2
T1sample_2isotropicsample_conditions_2
T2sample_2isotropicsample_conditions_2

Software:

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView v5.0, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Bruker Avance 400 MHz
  • Bruker Avance 700 MHz

Related Database Links:

PDB
EMBL CAJ19764 CAJ19808 CAJ19922 CCG39510 CEH38585
GB AAM39241 AAM39316 AAX12196 AAX12212 AGH79850
REF WP_003491212 YP_001965985 YP_001966014

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks