BMRB Entry 15868

Name: 1H, 13C and 15N resonance assignment of guanidine-denatured GED of dynamin
Published: 2008-07-24

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15868

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N resonance assignment of guanidine-denatured GED of dynamin

Deposition date:

2008-07-08

Original release date:

2008-07-24

Authors:

Chugh, Jeetender; Sharma, Shilpy; Hosur, Ramakrishna V

Citation:

Citation: Chugh, Jeetender; Sharma, Shilpy; Hosur, Ramakrishna V. "Pockets of short-range transient order and restricted topological heterogeneity in the guanidine-denatured state ensemble of GED of dynamin" Biochemistry 46, 11819-11832 (2007).
PubMed: 17910478

Assembly members:

Assembly members:
GED, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX4T1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GED: GSASFLRAGVYPERVGDKEK ASETEENGSDSFMHSMDPQL ERQVETIRNLVDSYMAIVNK TVRDLMPKTIMHLMINNTKE FIFSELLANLYSCGDQNTLM EESAEQAQRRDEMLRMYHAL KEALSIIGNINTTTVSTP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	380
15N chemical shifts	131
1H chemical shifts	569

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GED	1

Entities:

Entity 1, GED 138 residues - Formula weight is not available

1

GLY

SER

ALA

SER

PHE

LEU

ARG

ALA

GLY

VAL

2

TYR

PRO

GLU

ARG

VAL

GLY

ASP

LYS

GLU

LYS

3

ALA

SER

GLU

THR

GLU

ASN

GLY

SER

ASP

4

SER

PHE

MET

HIS

SER

MET

ASP

PRO

GLN

LEU

5

GLU

ARG

GLN

VAL

GLU

THR

ILE

ARG

ASN

LEU

6

VAL

ASP

SER

TYR

MET

ALA

ILE

VAL

ASN

LYS

7

THR

VAL

ARG

ASP

LEU

MET

PRO

LYS

THR

ILE

8

MET

HIS

LEU

MET

ILE

ASN

THR

LYS

GLU

9

PHE

ILE

PHE

SER

GLU

LEU

ALA

ASN

LEU

10

TYR

SER

CYS

GLY

ASP

GLN

ASN

THR

LEU

MET

11

GLU

SER

ALA

GLU

GLN

ALA

GLN

ARG

12

ASP

GLU

MET

LEU

ARG

MET

TYR

HIS

ALA

LEU

13

LYS

GLU

ALA

LEU

SER

ILE

GLY

ASN

ILE

14

ASN

THR

VAL

SER

THR

PRO

Samples:

15N_GED: GED, [U-100% 15N], 1 mM; DTT 1 mM; sodium acetate 10 mM; sodium chloride 150 mM; guanidine 6 M; EDTA 1 mM; H2O 90%; D2O, [U-100% 2H], 10%

15N_13C_GED: GED, [U-100% 13C; U-100% 15N], 1 mM; DTT 1 mM; sodium acetate 10 mM; sodium chloride 150 mM; guanidine 6 M; EDTA 1 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 150 mM; pH: 5.0; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	15N_GED	isotropic	sample_conditions_1
3D CBCA(CO)NH	15N_13C_GED	isotropic	sample_conditions_1
3D CBCANH	15N_13C_GED	isotropic	sample_conditions_1
3D HNCO	15N_13C_GED	isotropic	sample_conditions_1
3D HN(CA)CO	15N_13C_GED	isotropic	sample_conditions_1
3D 1H-15N NOESY	15N_GED	isotropic	sample_conditions_1
3D 1H-15N TOCSY	15N_GED	isotropic	sample_conditions_1
3D HNN	15N_13C_GED	isotropic	sample_conditions_1

Software:

FELIX, Accelrys Software Inc. - processing

CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Avance 800 MHz

BMRB	15867 16948
PDB	3SNH 3ZVR 4UUD 4UUK
DBJ	BAD90284 BAE25726 BAG53342 BAG58137 BAG59018
EMBL	CAA38397
GB	AAA02803 AAA37318 AAA37319 AAA37323 AAA37324
PIR	B40671
PRF	1614348A
REF	NP_001005336 NP_001070288 NP_001124521 NP_001275666 NP_001275667
SP	P21575 P39053 Q05193 Q08DF4
TPG	DAA24145
AlphaFold	P21575 P39053 Q05193 Q08DF4