BMRB Entry 15849

Name: SOLUTION NMR STRUCTURE OF the second OB-fold domain of replication protein A from Methanococcus maripaludis. NORTHEAST STRUCTURAL GENOMICS TARGET MrR110B.
Published: 2008-07-01

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PDB ID: 2k5v
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15849
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SOLUTION NMR STRUCTURE OF the second OB-fold domain of replication protein A from Methanococcus maripaludis. NORTHEAST STRUCTURAL GENOMICS TARGET MrR110B.

Deposition date:

2008-06-30

Original release date:

2008-07-01

Authors:

Aramini, James; Maglaqui, Melissa; Jiang, Mei; Ciccosanti, Colleen; Xiao, Rong; Nair, Rajesh; Everett, John; Swapna, G.; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano

Citation:

Citation: Aramini, James; Maglaqui, Melissa; Jiang, Mei; Ciccosanti, Colleen; Xiao, Rong; Nair, Rajesh; Everett, John; Swapna, G.; Acton, Thomas; Montelione, Gaetano. "SOLUTION NMR STRUCTURE OF the second OB-fold domain of replication protein A from Methanococcus maripaludis. NORTHEAST STRUCTURAL GENOMICS TARGET MrR110B." .

Assembly members:

Assembly members:
MrR110B, polymer, 104 residues, 11631.229 Da.

Natural source:

Natural source: Common Name: Methanococcus maripaludis Taxonomy ID: 39152 Superkingdom: Archaea Kingdom: not available Genus/species: Methanococcus maripaludis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: MrR110B-21.1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MrR110B: MNYKISELMPNLSGTINAEV VAAYPKKEFSRKDGTKGQLK SLFLKDDTGSIRGTLWNELA DFEVKKGDIAEVSGYVKQGY SGLEISVDNIGIIEKSLEHH HHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	450
15N chemical shifts	105
1H chemical shifts	708

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MrR110B	1

Entities:

Entity 1, MrR110B 104 residues - 11631.229 Da.

C-terminal LEHHHHHH purification tag.

1

MET

ASN

TYR

LYS

ILE

SER

GLU

LEU

MET

PRO

2

ASN

LEU

SER

GLY

THR

ILE

ASN

ALA

GLU

VAL

3

VAL

ALA

TYR

PRO

LYS

GLU

PHE

SER

4

ARG

LYS

ASP

GLY

THR

LYS

GLY

GLN

LEU

LYS

5

SER

LEU

PHE

LEU

LYS

ASP

THR

GLY

SER

6

ILE

ARG

GLY

THR

LEU

TRP

ASN

GLU

LEU

ALA

7

ASP

PHE

GLU

VAL

LYS

GLY

ASP

ILE

ALA

8

GLU

VAL

SER

GLY

TYR

VAL

LYS

GLN

GLY

TYR

9

SER

GLY

LEU

GLU

ILE

SER

VAL

ASP

ASN

ILE

10

GLY

ILE

GLU

LYS

SER

LEU

GLU

HIS

11

HIS

Samples:

sample_1: MrR110B, [U-100% 13C; U-100% 15N], 1.18 mM; MES 20 mM; sodium chloride 100 mM; calcium chloride 5 mM; DTT 10 mM; sodium azide 0.02%

sample_2: MrR110B, [U-100% 13C; U-100% 15N], 1.18 mM; MES 20 mM; sodium chloride 100 mM; calcium chloride 5 mM; DTT 10 mM; sodium azide 0.02%

sample_3: MrR110B, [U-5% 13C; U-100% 15N], 0.94 mM; MES 20 mM; sodium chloride 100 mM; calcium chloride 5 mM; DTT 10 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC (aliph)	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY aliphatic	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY aliphatic	sample_1	isotropic	sample_conditions_1
3D CCH-TOCSY aliphatic	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D simultaneous CN NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D simultaneous CN NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC high res. (L/V stereoassignment)	sample_3	isotropic	sample_conditions_1
2D 1H-15N hetNOE	sample_3	isotropic	sample_conditions_1
2D 1H-15N T1	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC (arom)	sample_1	isotropic	sample_conditions_1
2D 1H-15N T2	sample_3	isotropic	sample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection

AutoAssign v2.4.0, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

NMRPipe v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

SPARKY v3.113, Goddard - data analysis, peak picking

AutoStruct v2.2.1, Huang, Tejero, Powers and Montelione - RPF validation

PSVS v1.3, Bhattacharya and Montelione - structure validation

PDBStat v5.0, Tejero and Montelione - PDB analysis

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 600 MHz